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Improved DET communication between cellobiose dehydrogenase and a gold electrode modified with a rigid self-assembled monolayer and green metal nanoparticles : The role of an ordered nanostructuration

Bollella, Paolo; Mazzei, Franco; Favero, Gabriele; Fusco, Giovanni; Ludwig, R; Gorton, L. LU and Antiochia, Riccarda (2017) In Biosensors and Bioelectronics 88. p.196-203
Abstract

Efficient direct electron transfer (DET) between cellobiose dehydrogenase from Corynascus thermophilus (CtCDH) and a novel gold electrode platform, obtained by covalent linking of green AuNPs and AgNPs modified with a dithiol self-assembled monolayer, consisting of biphenyl-4,4′-dithiol (BPDT), was presented. The green AuNPs and AgNPs were synthesized using quercetin as reducing agent at room temperature. TEM experiments showed that the AuNPs and AgNPs were circular in shape with an average diameter of 5 and 8 nm, respectively. Cyclic voltammetry of CtCDH immobilized onto the AuNPs/BPDT/AuE and the AgNPs/BPDT/AuE electrode platforms were carried out and compared with naked AuE, BPDT/AuE, AuNPs/AuE, and AgNPs/AuE. A pair of well-defined... (More)

Efficient direct electron transfer (DET) between cellobiose dehydrogenase from Corynascus thermophilus (CtCDH) and a novel gold electrode platform, obtained by covalent linking of green AuNPs and AgNPs modified with a dithiol self-assembled monolayer, consisting of biphenyl-4,4′-dithiol (BPDT), was presented. The green AuNPs and AgNPs were synthesized using quercetin as reducing agent at room temperature. TEM experiments showed that the AuNPs and AgNPs were circular in shape with an average diameter of 5 and 8 nm, respectively. Cyclic voltammetry of CtCDH immobilized onto the AuNPs/BPDT/AuE and the AgNPs/BPDT/AuE electrode platforms were carried out and compared with naked AuE, BPDT/AuE, AuNPs/AuE, and AgNPs/AuE. A pair of well-defined redox waves in neutral pH solution due to efficient DET of CtCDH was present with both MNPs/BPDT/AuE platforms. No DET communication was found with platforms without MNPs linked to BPDT. The apparent heterogeneous electron transfer rate constants (kS) of CtCDH were calculated to be 21.5±0.8 s−1 and 10.3±0.7 s−1, for the AuNPs/BPDT/AuE and the AgNPs/BPDT/AuE platforms, respectively. The modified electrodes were successively used to develop an eco-friendly biosensor for lactose detection. The CtCDH/AuNPs/BPDT/AuE based biosensor showed the best analytical performances with an excellent stability, a detection limit of 3 µM, a linear range between 5 and 400 µM and a sensitivity of 27.5±2.5 µA cm−2 mM−1. Such performances were favorably compared with other lactose biosensors reported in literature. The biosensor was successively tested to quantify lactose content in real milk and cream samples. No significant interference present in the sample matrices was observed.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Biphenyl-4,4′-dithiol, Cellobiose dehydrogenase, Direct electron transfer, Gold nanoparticles, Lactose, Silver nanoparticles
in
Biosensors and Bioelectronics
volume
88
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84994507804
  • wos:000389112700027
ISSN
0956-5663
DOI
10.1016/j.bios.2016.08.027
language
English
LU publication?
yes
id
48f352eb-fcec-47ab-acbf-bb94d1e7a161
date added to LUP
2017-02-03 10:21:23
date last changed
2017-09-24 05:06:39
@article{48f352eb-fcec-47ab-acbf-bb94d1e7a161,
  abstract     = {<p>Efficient direct electron transfer (DET) between cellobiose dehydrogenase from Corynascus thermophilus (CtCDH) and a novel gold electrode platform, obtained by covalent linking of green AuNPs and AgNPs modified with a dithiol self-assembled monolayer, consisting of biphenyl-4,4′-dithiol (BPDT), was presented. The green AuNPs and AgNPs were synthesized using quercetin as reducing agent at room temperature. TEM experiments showed that the AuNPs and AgNPs were circular in shape with an average diameter of 5 and 8 nm, respectively. Cyclic voltammetry of CtCDH immobilized onto the AuNPs/BPDT/AuE and the AgNPs/BPDT/AuE electrode platforms were carried out and compared with naked AuE, BPDT/AuE, AuNPs/AuE, and AgNPs/AuE. A pair of well-defined redox waves in neutral pH solution due to efficient DET of CtCDH was present with both MNPs/BPDT/AuE platforms. No DET communication was found with platforms without MNPs linked to BPDT. The apparent heterogeneous electron transfer rate constants (k<sub>S</sub>) of CtCDH were calculated to be 21.5±0.8 s<sup>−1</sup> and 10.3±0.7 s<sup>−1</sup>, for the AuNPs/BPDT/AuE and the AgNPs/BPDT/AuE platforms, respectively. The modified electrodes were successively used to develop an eco-friendly biosensor for lactose detection. The CtCDH/AuNPs/BPDT/AuE based biosensor showed the best analytical performances with an excellent stability, a detection limit of 3 µM, a linear range between 5 and 400 µM and a sensitivity of 27.5±2.5 µA cm<sup>−2</sup> mM<sup>−1</sup>. Such performances were favorably compared with other lactose biosensors reported in literature. The biosensor was successively tested to quantify lactose content in real milk and cream samples. No significant interference present in the sample matrices was observed.</p>},
  author       = {Bollella, Paolo and Mazzei, Franco and Favero, Gabriele and Fusco, Giovanni and Ludwig, R and Gorton, L. and Antiochia, Riccarda},
  issn         = {0956-5663},
  keyword      = {Biphenyl-4,4′-dithiol,Cellobiose dehydrogenase,Direct electron transfer,Gold nanoparticles,Lactose,Silver nanoparticles},
  language     = {eng},
  month        = {02},
  pages        = {196--203},
  publisher    = {Elsevier},
  series       = {Biosensors and Bioelectronics},
  title        = {Improved DET communication between cellobiose dehydrogenase and a gold electrode modified with a rigid self-assembled monolayer and green metal nanoparticles : The role of an ordered nanostructuration},
  url          = {http://dx.doi.org/10.1016/j.bios.2016.08.027},
  volume       = {88},
  year         = {2017},
}