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Precision and sensitivity of the measurement of 15 N enrichment in D-alanine from bacterial cell walls using positive/negative ion mass spectrometry

Tunlid, Anders LU ; Odham, Göran ; Findlay, Robert H. and White, David C. (1985) In Journal of Microbiological Methods 3(3-4). p.237-245
Abstract


Sensitive detection of cellular components from specific groups of microbes can be utilized as 'signatures' in the examination of microbial consortia from soils, sediments or biofilms. Utilizing capillary gas chromatography/mass spectrometry and stereospecific derivatizing agents, D-alanine, a component localized in the prokaryotic (bacteria) cell wall, can be detected reproducibly. Enrichments of D-
15
N]alanine determined in E. coli grown with [
15
N]ammonia can be determined with precision at 1.0 atom%. Chemical ionization with methane gas and the... (More)


Sensitive detection of cellular components from specific groups of microbes can be utilized as 'signatures' in the examination of microbial consortia from soils, sediments or biofilms. Utilizing capillary gas chromatography/mass spectrometry and stereospecific derivatizing agents, D-alanine, a component localized in the prokaryotic (bacteria) cell wall, can be detected reproducibly. Enrichments of D-
15
N]alanine determined in E. coli grown with [
15
N]ammonia can be determined with precision at 1.0 atom%. Chemical ionization with methane gas and the detection of negative ions (M-HF)
-
· and (M-F or M+H - HF)
-
formed from the heptafluorobutyryl D-2 butanol ester of D-alanine allowed as little as 8 pg (90 fmol) to be detected reproducibly. This method can be utilized to define the metabolic activity in terms of
15
N incorporation at the level of 10
3
-10
4
cells, as a function of the
15
N-
14
N ratio.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacterial cell wall, Capillary gas chromatography/mass spectrometry, D-Alanine, Positive/negative ion chemical ionization, Selected ion monitoring, Stable isotope labeling
in
Journal of Microbiological Methods
volume
3
issue
3-4
pages
237 - 245
publisher
Elsevier
external identifiers
  • pmid:11539051
  • scopus:0021888551
ISSN
0167-7012
DOI
10.1016/0167-7012(85)90051-X
language
English
LU publication?
yes
id
48fd1f95-c50e-4d03-9c03-37a3ef9202eb
date added to LUP
2019-10-23 17:35:44
date last changed
2025-01-10 01:13:45
@article{48fd1f95-c50e-4d03-9c03-37a3ef9202eb,
  abstract     = {{<p><br>
                            Sensitive detection of cellular components from specific groups of microbes can be utilized as 'signatures' in the examination of microbial consortia from soils, sediments or biofilms. Utilizing capillary gas chromatography/mass spectrometry and stereospecific derivatizing agents, D-alanine, a component localized in the prokaryotic (bacteria) cell wall, can be detected reproducibly. Enrichments of D-<br>
                            <sup>15</sup><br>
                            N]alanine determined in E. coli grown with [<br>
                            <sup>15</sup><br>
                            N]ammonia can be determined with precision at 1.0 atom%. Chemical ionization with methane gas and the detection of negative ions (M-HF)<br>
                            <sup>-</sup><br>
                            · and (M-F or M+H - HF)<br>
                            <sup>-</sup><br>
                             formed from the heptafluorobutyryl D-2 butanol ester of D-alanine allowed as little as 8 pg (90 fmol) to be detected reproducibly. This method can be utilized to define the metabolic activity in terms of <br>
                            <sup>15</sup><br>
                            N incorporation at the level of 10<br>
                            <sup>3</sup><br>
                            -10<br>
                            <sup>4</sup><br>
                             cells, as a function of the <br>
                            <sup>15</sup><br>
                            N-<br>
                            <sup>14</sup><br>
                            N ratio.</p>}},
  author       = {{Tunlid, Anders and Odham, Göran and Findlay, Robert H. and White, David C.}},
  issn         = {{0167-7012}},
  keywords     = {{Bacterial cell wall; Capillary gas chromatography/mass spectrometry; D-Alanine; Positive/negative ion chemical ionization; Selected ion monitoring; Stable isotope labeling}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3-4}},
  pages        = {{237--245}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Microbiological Methods}},
  title        = {{Precision and sensitivity of the measurement of 
                        <sup>15</sup>
                        N enrichment in D-alanine from bacterial cell walls using positive/negative ion mass spectrometry}},
  url          = {{http://dx.doi.org/10.1016/0167-7012(85)90051-X}},
  doi          = {{10.1016/0167-7012(85)90051-X}},
  volume       = {{3}},
  year         = {{1985}},
}