Characterizing the Composition of Bone Formed During Fracture Healing Using Scanning Electron Microscopy Techniques.
Perdikouri, Christina LU ; Tägil, Magnus LU and Isaksson, Hanna LU
(2015)
In Calcified Tissue International
96(1).
p.11-17
- Abstract
- About 5-10 % of all bone fractures suffer from delayed healing, which may lead to non-union. Bone morphogenetic proteins (BMPs) can be used to induce differentiation of osteoblasts and enhance the formation of the bony callus, and bisphosphonates help to retain the newly formed callus. The aim of this study was to investigate if scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) can identify differences in the mineral composition of the newly formed bone compared to cortical bone from a non-fractured control. Moreover, we investigate whether the use of BMPs and bisphosphonates-alone or combined-may have an effect on bone mineralization and composition. Twelve male Sprague-Dawley rats at 9 weeks of age were... (More)
- About 5-10 % of all bone fractures suffer from delayed healing, which may lead to non-union. Bone morphogenetic proteins (BMPs) can be used to induce differentiation of osteoblasts and enhance the formation of the bony callus, and bisphosphonates help to retain the newly formed callus. The aim of this study was to investigate if scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) can identify differences in the mineral composition of the newly formed bone compared to cortical bone from a non-fractured control. Moreover, we investigate whether the use of BMPs and bisphosphonates-alone or combined-may have an effect on bone mineralization and composition. Twelve male Sprague-Dawley rats at 9 weeks of age were randomly divided into four groups and treated with (A) saline, (B) BMP-7, (C) bisphosphonates (Zoledronate), and (D) BMP-7 + Zoledronate. The rats were sacrificed after 6 weeks. All samples were imaged using SEM and chemically analyzed with EDS to quantify the amount of C, N, Ca, P, O, Na, and Mg. The Ca/P ratio was the primary outcome. In the fractured samples, two areas of interest were chosen for chemical analysis with EDS: the callus and the cortical bone. In the non-fractured samples, only the cortex was analyzed. Our results showed that the element composition varied to a small extent between the callus and the cortical bone in the fractured bones. However, the Ca/P ratio did not differ significantly, suggesting that the mineralization at all sites is similar 6 weeks post-fracture in this rat model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4905681
- author
- Perdikouri, Christina
LU
; Tägil, Magnus
LU
and Isaksson, Hanna
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Calcified Tissue International
- volume
- 96
- issue
- 1
- pages
- 11 - 17
- publisher
- Springer
- external identifiers
-
- pmid:25537634
- wos:000347709400002
- scopus:84926621165
- pmid:25537634
- ISSN
- 1432-0827
- DOI
- 10.1007/s00223-014-9930-z
- language
- English
- LU publication?
- yes
- id
- b1ea32c8-2f6d-43a4-b094-63bc5cbcc5c0 (old id 4905681)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25537634?dopt=Abstract
- date added to LUP
- 2016-04-01 10:31:37
- date last changed
- 2025-01-14 17:00:21
@article{b1ea32c8-2f6d-43a4-b094-63bc5cbcc5c0,
abstract = {{About 5-10 % of all bone fractures suffer from delayed healing, which may lead to non-union. Bone morphogenetic proteins (BMPs) can be used to induce differentiation of osteoblasts and enhance the formation of the bony callus, and bisphosphonates help to retain the newly formed callus. The aim of this study was to investigate if scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) can identify differences in the mineral composition of the newly formed bone compared to cortical bone from a non-fractured control. Moreover, we investigate whether the use of BMPs and bisphosphonates-alone or combined-may have an effect on bone mineralization and composition. Twelve male Sprague-Dawley rats at 9 weeks of age were randomly divided into four groups and treated with (A) saline, (B) BMP-7, (C) bisphosphonates (Zoledronate), and (D) BMP-7 + Zoledronate. The rats were sacrificed after 6 weeks. All samples were imaged using SEM and chemically analyzed with EDS to quantify the amount of C, N, Ca, P, O, Na, and Mg. The Ca/P ratio was the primary outcome. In the fractured samples, two areas of interest were chosen for chemical analysis with EDS: the callus and the cortical bone. In the non-fractured samples, only the cortex was analyzed. Our results showed that the element composition varied to a small extent between the callus and the cortical bone in the fractured bones. However, the Ca/P ratio did not differ significantly, suggesting that the mineralization at all sites is similar 6 weeks post-fracture in this rat model.}},
author = {{Perdikouri, Christina and Tägil, Magnus and Isaksson, Hanna}},
issn = {{1432-0827}},
language = {{eng}},
number = {{1}},
pages = {{11--17}},
publisher = {{Springer}},
series = {{Calcified Tissue International}},
title = {{Characterizing the Composition of Bone Formed During Fracture Healing Using Scanning Electron Microscopy Techniques.}},
url = {{http://dx.doi.org/10.1007/s00223-014-9930-z}},
doi = {{10.1007/s00223-014-9930-z}},
volume = {{96}},
year = {{2015}},
}