Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow.
(2014) In Stem Cell Reports 3(6). p.965-974- Abstract
- Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-)... (More)
- Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4913145
- author
- Li, Hongzhe LU ; Ghazanfari, Roshanak LU ; Zacharaki, Dimitra LU ; Ditzel, Nicholas ; Isern, Joan ; Ekblom, Marja LU ; Méndez-Ferrer, Simón ; Kassem, Moustapha and Scheding, Stefan LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Reports
- volume
- 3
- issue
- 6
- pages
- 965 - 974
- publisher
- Cell Press
- external identifiers
-
- pmid:25454633
- wos:000346159300005
- scopus:84920502326
- pmid:25454633
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2014.09.018
- language
- English
- LU publication?
- yes
- id
- 592bdf79-ecd7-4d4d-b652-fc84b4197717 (old id 4913145)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25454633?dopt=Abstract
- date added to LUP
- 2016-04-01 13:03:10
- date last changed
- 2024-03-12 22:07:36
@article{592bdf79-ecd7-4d4d-b652-fc84b4197717, abstract = {{Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.}}, author = {{Li, Hongzhe and Ghazanfari, Roshanak and Zacharaki, Dimitra and Ditzel, Nicholas and Isern, Joan and Ekblom, Marja and Méndez-Ferrer, Simón and Kassem, Moustapha and Scheding, Stefan}}, issn = {{2213-6711}}, language = {{eng}}, number = {{6}}, pages = {{965--974}}, publisher = {{Cell Press}}, series = {{Stem Cell Reports}}, title = {{Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow.}}, url = {{https://lup.lub.lu.se/search/files/3133546/7859390.pdf}}, doi = {{10.1016/j.stemcr.2014.09.018}}, volume = {{3}}, year = {{2014}}, }