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Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow.

Li, Hongzhe LU ; Ghazanfari, Roshanak LU ; Zacharaki, Dimitra LU ; Ditzel, Nicholas; Isern, Joan; Ekblom, Marja LU ; Méndez-Ferrer, Simón; Kassem, Moustapha and Scheding, Stefan LU (2014) In Stem Cell Reports 3(6). p.965-974
Abstract
Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-)... (More)
Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity. (Less)
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type
Contribution to journal
publication status
published
subject
in
Stem Cell Reports
volume
3
issue
6
pages
965 - 974
publisher
Cell Press
external identifiers
  • pmid:25454633
  • wos:000346159300005
  • scopus:84920502326
ISSN
2213-6711
DOI
10.1016/j.stemcr.2014.09.018
language
English
LU publication?
yes
id
592bdf79-ecd7-4d4d-b652-fc84b4197717 (old id 4913145)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25454633?dopt=Abstract
date added to LUP
2015-01-08 16:16:46
date last changed
2017-10-01 04:02:31
@article{592bdf79-ecd7-4d4d-b652-fc84b4197717,
  abstract     = {Human bone marrow (BM) contains a rare population of nonhematopoietic mesenchymal stromal cells (MSCs), which are of central importance for the hematopoietic microenvironment. However, the precise phenotypic definition of these cells in adult BM has not yet been reported. In this study, we show that low/negative expression of CD140a (PDGFR-α) on lin(-)/CD45(-)/CD271(+) BM cells identified a cell population with very high MSC activity, measured as fibroblastic colony-forming unit frequency and typical in vitro and in vivo stroma formation and differentiation capacities. Furthermore, these cells exhibited high levels of genes associated with mesenchymal lineages and HSC supportive function. Moreover, lin(-)/CD45(-)/CD271(+)/CD140a(low/-) cells effectively mediated the ex vivo expansion of transplantable CD34(+) hematopoietic stem cells. Taken together, these data indicate that CD140a is a key negative selection marker for adult human BM-MSCs, which enables to prospectively isolate a close to pure population of candidate human adult stroma stem/progenitor cells with potent hematopoiesis-supporting capacity.},
  author       = {Li, Hongzhe and Ghazanfari, Roshanak and Zacharaki, Dimitra and Ditzel, Nicholas and Isern, Joan and Ekblom, Marja and Méndez-Ferrer, Simón and Kassem, Moustapha and Scheding, Stefan},
  issn         = {2213-6711},
  language     = {eng},
  number       = {6},
  pages        = {965--974},
  publisher    = {Cell Press},
  series       = {Stem Cell Reports},
  title        = {Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow.},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2014.09.018},
  volume       = {3},
  year         = {2014},
}