A method for time-resolved calorespirometry of terrestrial samples.
(2015) In Methods 76(Online 23 October 2014). p.20-26- Abstract
- A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide... (More)
- A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4913294
- author
- Wadsö, Lars LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods
- volume
- 76
- issue
- Online 23 October 2014
- pages
- 20 - 26
- publisher
- Elsevier
- external identifiers
-
- pmid:25449900
- wos:000352332000004
- scopus:84925397250
- pmid:25449900
- ISSN
- 1095-9130
- DOI
- 10.1016/j.ymeth.2014.10.001
- language
- English
- LU publication?
- yes
- id
- 76fa10a7-6c3e-4b5f-878f-481d2b954cb4 (old id 4913294)
- date added to LUP
- 2016-04-01 10:37:17
- date last changed
- 2022-01-26 00:56:27
@article{76fa10a7-6c3e-4b5f-878f-481d2b954cb4, abstract = {{A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed.}}, author = {{Wadsö, Lars}}, issn = {{1095-9130}}, language = {{eng}}, number = {{Online 23 October 2014}}, pages = {{20--26}}, publisher = {{Elsevier}}, series = {{Methods}}, title = {{A method for time-resolved calorespirometry of terrestrial samples.}}, url = {{http://dx.doi.org/10.1016/j.ymeth.2014.10.001}}, doi = {{10.1016/j.ymeth.2014.10.001}}, volume = {{76}}, year = {{2015}}, }