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A method for time-resolved calorespirometry of terrestrial samples.

Wadsö, Lars LU (2015) In Methods 76(Online 23 October 2014). p.20-26
Abstract
A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide... (More)
A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods
volume
76
issue
Online 23 October 2014
pages
20 - 26
publisher
Elsevier
external identifiers
  • pmid:25449900
  • wos:000352332000004
  • scopus:84925397250
ISSN
1095-9130
DOI
10.1016/j.ymeth.2014.10.001
language
English
LU publication?
yes
id
76fa10a7-6c3e-4b5f-878f-481d2b954cb4 (old id 4913294)
date added to LUP
2015-01-13 15:44:04
date last changed
2017-02-26 03:13:43
@article{76fa10a7-6c3e-4b5f-878f-481d2b954cb4,
  abstract     = {A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (&lt;1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed.},
  author       = {Wadsö, Lars},
  issn         = {1095-9130},
  language     = {eng},
  number       = {Online 23 October 2014},
  pages        = {20--26},
  publisher    = {Elsevier},
  series       = {Methods},
  title        = {A method for time-resolved calorespirometry of terrestrial samples.},
  url          = {http://dx.doi.org/10.1016/j.ymeth.2014.10.001},
  volume       = {76},
  year         = {2015},
}