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Analytical platforms in vitreoretinal proteomics

Cehofski, Lasse Jorgensen; Mandal, Nakul LU ; Honore, Bent and Vorum, Henrik (2014) In Bioanalysis 6(22). p.3051-3066
Abstract
Current proteomic technologies can effectively be used to study the proteins of the vitreous body and retina in health and disease. The use of appropriate samples, analytical platform and bioinformatic method are essential factors to consider when undertaking such studies. Certain proteins may hinder the detection and evaluation of more relevant proteins associated with pathological processes if not carefully considered, particularly in the sample preparation and data analysis stages. The utilization of more than one quantification technique and database search program to expand the level of proteome coverage and analysis will help to generate more robust and worthwhile results. This review discusses important aspects of sample processing... (More)
Current proteomic technologies can effectively be used to study the proteins of the vitreous body and retina in health and disease. The use of appropriate samples, analytical platform and bioinformatic method are essential factors to consider when undertaking such studies. Certain proteins may hinder the detection and evaluation of more relevant proteins associated with pathological processes if not carefully considered, particularly in the sample preparation and data analysis stages. The utilization of more than one quantification technique and database search program to expand the level of proteome coverage and analysis will help to generate more robust and worthwhile results. This review discusses important aspects of sample processing and the use of label and label-free quantitative proteomics strategies applied to the vitreous and retina. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioanalysis
volume
6
issue
22
pages
3051 - 3066
publisher
Future Science
external identifiers
  • wos:000346242300010
  • scopus:84918589570
ISSN
1757-6180
DOI
10.4155/bio.14.227
language
English
LU publication?
yes
id
f95eb9b7-5afb-451d-a317-ad8444069fb3 (old id 4950614)
date added to LUP
2015-02-03 07:05:41
date last changed
2017-11-05 03:06:34
@article{f95eb9b7-5afb-451d-a317-ad8444069fb3,
  abstract     = {Current proteomic technologies can effectively be used to study the proteins of the vitreous body and retina in health and disease. The use of appropriate samples, analytical platform and bioinformatic method are essential factors to consider when undertaking such studies. Certain proteins may hinder the detection and evaluation of more relevant proteins associated with pathological processes if not carefully considered, particularly in the sample preparation and data analysis stages. The utilization of more than one quantification technique and database search program to expand the level of proteome coverage and analysis will help to generate more robust and worthwhile results. This review discusses important aspects of sample processing and the use of label and label-free quantitative proteomics strategies applied to the vitreous and retina.},
  author       = {Cehofski, Lasse Jorgensen and Mandal, Nakul and Honore, Bent and Vorum, Henrik},
  issn         = {1757-6180},
  language     = {eng},
  number       = {22},
  pages        = {3051--3066},
  publisher    = {Future Science},
  series       = {Bioanalysis},
  title        = {Analytical platforms in vitreoretinal proteomics},
  url          = {http://dx.doi.org/10.4155/bio.14.227},
  volume       = {6},
  year         = {2014},
}