Advanced

In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D)

Quan, Xueling; Heiskanen, Arto; Tenje, Maria LU and Boisen, Anja (2014) In Electrochemistry Communications 48. p.111-114
Abstract
The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (E-pzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results, we present a model to explain the influence of the potential pulsing.... (More)
The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (E-pzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results, we present a model to explain the influence of the potential pulsing. Aside from the effect of applied potentials on DNA related processes, this work also demonstrates the versatility of the combination of electrochemistry and quartz crystal microbalance with dissipation in facilitating real-time in situ monitoring of such processes. (C) 2014 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Potential pulse assisted functionalization, Thiolated DNA, functionalization, DNA hybridization, Vertically aligned DNA, conformation, EQCM-D
in
Electrochemistry Communications
volume
48
pages
111 - 114
publisher
Elsevier
external identifiers
  • wos:000345208000028
  • scopus:84907469779
ISSN
1388-2481
DOI
10.1016/j.elecom.2014.08.029
language
English
LU publication?
yes
id
596db658-6ed0-4d33-9929-7059c3d50b46 (old id 4984599)
date added to LUP
2015-01-27 09:09:25
date last changed
2017-01-01 03:39:44
@article{596db658-6ed0-4d33-9929-7059c3d50b46,
  abstract     = {The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (E-pzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results, we present a model to explain the influence of the potential pulsing. Aside from the effect of applied potentials on DNA related processes, this work also demonstrates the versatility of the combination of electrochemistry and quartz crystal microbalance with dissipation in facilitating real-time in situ monitoring of such processes. (C) 2014 Elsevier B.V. All rights reserved.},
  author       = {Quan, Xueling and Heiskanen, Arto and Tenje, Maria and Boisen, Anja},
  issn         = {1388-2481},
  keyword      = {Potential pulse assisted functionalization,Thiolated DNA,functionalization,DNA hybridization,Vertically aligned DNA,conformation,EQCM-D},
  language     = {eng},
  pages        = {111--114},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D)},
  url          = {http://dx.doi.org/10.1016/j.elecom.2014.08.029},
  volume       = {48},
  year         = {2014},
}