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Microsatellite markers for population genetic studies of the rock firefinch, Lagonosticta sanguinodorsalis

Abalaka, Jacinta LU and Hansson, Bengt LU (2014) In African Zoology 49(2). p.301-306
Abstract
We identified the first set of microsatellites for use in the rock firefinch (Lagonosticta sanguinodorsalis). First, we tested existing passerine microsatellite loci for cross-species amplification success in a small sample of rock firefinches and identified 10 loci that were seemingly polymorphic and easy to score. Secondly, we developed and characterized three microsatellite loci de novo from the rock firefinch genome. In a larger sample of individuals from three populations, one locus initially interpreted as polymorphic was monomorphic. Among the polymorphic loci, the number of alleles ranged between 2 and 15 and the expected heterozygosity between 0.08 and 0.91 within populations. Two loci had high estimated null allele frequency in... (More)
We identified the first set of microsatellites for use in the rock firefinch (Lagonosticta sanguinodorsalis). First, we tested existing passerine microsatellite loci for cross-species amplification success in a small sample of rock firefinches and identified 10 loci that were seemingly polymorphic and easy to score. Secondly, we developed and characterized three microsatellite loci de novo from the rock firefinch genome. In a larger sample of individuals from three populations, one locus initially interpreted as polymorphic was monomorphic. Among the polymorphic loci, the number of alleles ranged between 2 and 15 and the expected heterozygosity between 0.08 and 0.91 within populations. Two loci had high estimated null allele frequency in at least one population. The microsatellites amplified very well (80% success) in five other African finch species and each locus was confirmed as polymorphic in at least one species. We conclude that these microsatellite markers will be useful for population genetic studies in the rock firefinch and other closely related African finches. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
population genetics, microsatellite, primer, Lagonosticta, sanguinodorsalis
in
African Zoology
volume
49
issue
2
pages
301 - 306
publisher
Zoological Soc Southern Africa
external identifiers
  • wos:000345228200016
  • scopus:84908616030
ISSN
1562-7020
DOI
10.3377/004.049.0202
project
Conservation of threatened populations
language
English
LU publication?
yes
id
12992e51-af28-486c-98fc-70cce11068d6 (old id 4985658)
date added to LUP
2015-01-27 08:34:06
date last changed
2017-01-01 03:52:41
@article{12992e51-af28-486c-98fc-70cce11068d6,
  abstract     = {We identified the first set of microsatellites for use in the rock firefinch (Lagonosticta sanguinodorsalis). First, we tested existing passerine microsatellite loci for cross-species amplification success in a small sample of rock firefinches and identified 10 loci that were seemingly polymorphic and easy to score. Secondly, we developed and characterized three microsatellite loci de novo from the rock firefinch genome. In a larger sample of individuals from three populations, one locus initially interpreted as polymorphic was monomorphic. Among the polymorphic loci, the number of alleles ranged between 2 and 15 and the expected heterozygosity between 0.08 and 0.91 within populations. Two loci had high estimated null allele frequency in at least one population. The microsatellites amplified very well (80% success) in five other African finch species and each locus was confirmed as polymorphic in at least one species. We conclude that these microsatellite markers will be useful for population genetic studies in the rock firefinch and other closely related African finches.},
  author       = {Abalaka, Jacinta and Hansson, Bengt},
  issn         = {1562-7020},
  keyword      = {population genetics,microsatellite,primer,Lagonosticta,sanguinodorsalis},
  language     = {eng},
  number       = {2},
  pages        = {301--306},
  publisher    = {Zoological Soc Southern Africa},
  series       = {African Zoology},
  title        = {Microsatellite markers for population genetic studies of the rock firefinch, Lagonosticta sanguinodorsalis},
  url          = {http://dx.doi.org/10.3377/004.049.0202},
  volume       = {49},
  year         = {2014},
}