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Sex influences DNA methylation and gene expression in human skeletal muscle myoblasts and myotubes

Davegårdh, Cajsa LU ; Hall Wedin, Elin LU ; Broholm, Christa; Henriksen, Tora Ida; Pedersen, Maria; Pedersen, Bente Klarlund; Scheele, Camilla and Ling, Charlotte LU (2019) In Stem Cell Research and Therapy 10(1).
Abstract

Background: Sex differences are known to impact muscle phenotypes, metabolism, and disease risk. Skeletal muscle stem cells (satellite cells) are important for muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts (activated satellite cells) before and after differentiation into myotubes. Method: We used an array-based approach to analyse genome-wide DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay. Results: Genome-wide DNA methylation and gene expression differences between the sexes were detected in both myoblasts and... (More)

Background: Sex differences are known to impact muscle phenotypes, metabolism, and disease risk. Skeletal muscle stem cells (satellite cells) are important for muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts (activated satellite cells) before and after differentiation into myotubes. Method: We used an array-based approach to analyse genome-wide DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay. Results: Genome-wide DNA methylation and gene expression differences between the sexes were detected in both myoblasts and myotubes, on the autosomes as well as the X-chromosome, despite lack of exposure to sex hormones and other factors that differ between sexes. Pathway analysis revealed higher expression of oxidative phosphorylation and other metabolic pathways in myoblasts from women compared to men. Oxidative phosphorylation was also enriched among genes with higher expression in myotubes from women. Forty genes in myoblasts and 9 in myotubes had differences in both DNA methylation and gene expression between the sexes, including LAMP2 and SIRT1 in myoblasts and KDM6A in myotubes. Furthermore, increased DNA methylation of LAMP2 promoter had negative effects on reporter gene expression. Five genes (CREB5, RPS4X, SYAP1, XIST, and ZRSR2) showed differential DNA methylation and gene expression between the sexes in both myoblasts and myotubes. Interestingly, differences in DNA methylation and expression between women and men were also found during differentiation (myoblasts versus myotubes), e.g., in genes involved in energy metabolism. Interestingly, more DNA methylation changes occur in women compared to men on autosomes. Conclusion: All together, we show that epigenetic and transcriptional differences exist in human myoblasts and myotubes as well as during differentiation between women and men. We believe that these intrinsic differences might contribute to sex dependent differences in muscular phenotypes.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Epigenetics, Epigenome, Gender, mRNA expression, Muscle stem cells, Myoblasts, Myogenesis, Myotubes, Skeletal muscle, Transcriptome
in
Stem Cell Research and Therapy
volume
10
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85060029288
ISSN
1757-6512
DOI
10.1186/s13287-018-1118-4
language
English
LU publication?
yes
id
499f9697-0c63-4429-a844-7d08d0035fea
date added to LUP
2019-01-29 08:21:17
date last changed
2019-11-13 05:25:30
@article{499f9697-0c63-4429-a844-7d08d0035fea,
  abstract     = {<p>Background: Sex differences are known to impact muscle phenotypes, metabolism, and disease risk. Skeletal muscle stem cells (satellite cells) are important for muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts (activated satellite cells) before and after differentiation into myotubes. Method: We used an array-based approach to analyse genome-wide DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay. Results: Genome-wide DNA methylation and gene expression differences between the sexes were detected in both myoblasts and myotubes, on the autosomes as well as the X-chromosome, despite lack of exposure to sex hormones and other factors that differ between sexes. Pathway analysis revealed higher expression of oxidative phosphorylation and other metabolic pathways in myoblasts from women compared to men. Oxidative phosphorylation was also enriched among genes with higher expression in myotubes from women. Forty genes in myoblasts and 9 in myotubes had differences in both DNA methylation and gene expression between the sexes, including LAMP2 and SIRT1 in myoblasts and KDM6A in myotubes. Furthermore, increased DNA methylation of LAMP2 promoter had negative effects on reporter gene expression. Five genes (CREB5, RPS4X, SYAP1, XIST, and ZRSR2) showed differential DNA methylation and gene expression between the sexes in both myoblasts and myotubes. Interestingly, differences in DNA methylation and expression between women and men were also found during differentiation (myoblasts versus myotubes), e.g., in genes involved in energy metabolism. Interestingly, more DNA methylation changes occur in women compared to men on autosomes. Conclusion: All together, we show that epigenetic and transcriptional differences exist in human myoblasts and myotubes as well as during differentiation between women and men. We believe that these intrinsic differences might contribute to sex dependent differences in muscular phenotypes.</p>},
  articleno    = {26},
  author       = {Davegårdh, Cajsa and Hall Wedin, Elin and Broholm, Christa and Henriksen, Tora Ida and Pedersen, Maria and Pedersen, Bente Klarlund and Scheele, Camilla and Ling, Charlotte},
  issn         = {1757-6512},
  keyword      = {Epigenetics,Epigenome,Gender,mRNA expression,Muscle stem cells,Myoblasts,Myogenesis,Myotubes,Skeletal muscle,Transcriptome},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Stem Cell Research and Therapy},
  title        = {Sex influences DNA methylation and gene expression in human skeletal muscle myoblasts and myotubes},
  url          = {http://dx.doi.org/10.1186/s13287-018-1118-4},
  volume       = {10},
  year         = {2019},
}