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Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host

Wille, Michelle; Tolf, Conny; Avril, Alexis; Latorre-Margalef, Neus LU ; Wallerström, Sofie; Olsen, Björn and Waldenström, Jonas LU (2013) In Virology 443(1). p.150-160
Abstract

Influenza A viruses (IAV) can dramatically alter both genotype and phenotype at a rapid rate as a product of co-infection and reassortment. Avian IAV exhibit high levels of phylogenetic incongruence, suggesting high levels of reassortment in the virus reservoir. Using a natural-experimental system, we reconstructed relationships amongst 92 viruses across 15 subtypes from 10 Mallards in an autumn season. Phylogenetic analyses estimated that 56% of the isolated viruses were reassorted. Network analysis demonstrated different patterns of reassortment and limited exchange of segments between primary and secondary infections. No clear patterns of linkage between segments were found, and patterns within a season were likely the consequence of... (More)

Influenza A viruses (IAV) can dramatically alter both genotype and phenotype at a rapid rate as a product of co-infection and reassortment. Avian IAV exhibit high levels of phylogenetic incongruence, suggesting high levels of reassortment in the virus reservoir. Using a natural-experimental system, we reconstructed relationships amongst 92 viruses across 15 subtypes from 10 Mallards in an autumn season. Phylogenetic analyses estimated that 56% of the isolated viruses were reassorted. Network analysis demonstrated different patterns of reassortment and limited exchange of segments between primary and secondary infections. No clear patterns of linkage between segments were found, and patterns within a season were likely the consequence of continued introduction of new constellations, high viral load and diversity in the wild bird reservoir, and co-infections. This is the first IAV study to implement multiple tools available for elucidating factors governing reassortment patterns in naturally infected Mallards.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anas platyrhynchos, Evolution, Influenza A virus, Mallard, Reassortment, Sentinel ducks
in
Virology
volume
443
issue
1
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:84879784330
ISSN
0042-6822
DOI
10.1016/j.virol.2013.05.004
language
English
LU publication?
no
id
49daecad-cd20-433a-a717-747480ae94d4
date added to LUP
2017-04-11 14:01:49
date last changed
2018-03-18 05:11:33
@article{49daecad-cd20-433a-a717-747480ae94d4,
  abstract     = {<p>Influenza A viruses (IAV) can dramatically alter both genotype and phenotype at a rapid rate as a product of co-infection and reassortment. Avian IAV exhibit high levels of phylogenetic incongruence, suggesting high levels of reassortment in the virus reservoir. Using a natural-experimental system, we reconstructed relationships amongst 92 viruses across 15 subtypes from 10 Mallards in an autumn season. Phylogenetic analyses estimated that 56% of the isolated viruses were reassorted. Network analysis demonstrated different patterns of reassortment and limited exchange of segments between primary and secondary infections. No clear patterns of linkage between segments were found, and patterns within a season were likely the consequence of continued introduction of new constellations, high viral load and diversity in the wild bird reservoir, and co-infections. This is the first IAV study to implement multiple tools available for elucidating factors governing reassortment patterns in naturally infected Mallards.</p>},
  author       = {Wille, Michelle and Tolf, Conny and Avril, Alexis and Latorre-Margalef, Neus and Wallerström, Sofie and Olsen, Björn and Waldenström, Jonas},
  issn         = {0042-6822},
  keyword      = {Anas platyrhynchos,Evolution,Influenza A virus,Mallard,Reassortment,Sentinel ducks},
  language     = {eng},
  month        = {08},
  number       = {1},
  pages        = {150--160},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Frequency and patterns of reassortment in natural influenza A virus infection in a reservoir host},
  url          = {http://dx.doi.org/10.1016/j.virol.2013.05.004},
  volume       = {443},
  year         = {2013},
}