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Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds

Jakobsson, Albin LU ; Ottosson, Maximilian LU ; Zalis, Marina Castro LU ; O'Carroll, David LU ; Johansson, Ulrica Englund LU and Johansson, Per Fredrik LU (2017) In Nanomedicine: Nanotechnology, Biology, and Medicine
Abstract

We demonstrate an artificial three-dimensional (3D) electrical active human neuronal network system, by the growth of brain neural progenitors in highly porous low density electrospun poly-ε-caprolactone (PCL) fiber scaffolds. In neuroscience research cell-based assays are important experimental instruments for studying neuronal function in health and disease. Traditional cell culture at 2D-surfaces induces abnormal cell-cell contacts and network formation. Hence, there is a tremendous need to explore in vivo-resembling 3D neural cell culture approaches. We present an improved electrospinning method for fabrication of scaffolds that promote neuronal differentiation into highly 3D integrated networks, formation of inhibitory and... (More)

We demonstrate an artificial three-dimensional (3D) electrical active human neuronal network system, by the growth of brain neural progenitors in highly porous low density electrospun poly-ε-caprolactone (PCL) fiber scaffolds. In neuroscience research cell-based assays are important experimental instruments for studying neuronal function in health and disease. Traditional cell culture at 2D-surfaces induces abnormal cell-cell contacts and network formation. Hence, there is a tremendous need to explore in vivo-resembling 3D neural cell culture approaches. We present an improved electrospinning method for fabrication of scaffolds that promote neuronal differentiation into highly 3D integrated networks, formation of inhibitory and excitatory synapses and extensive neurite growth. Notably, in 3D scaffolds in vivo-resembling intermixed neuronal and glial cell network were formed, whereas in parallel 2D cultures a neuronal cell layer grew separated from an underlying glial cell layer. Hence, the use of the 3D cell assay presented will most likely provide more physiological relevant results.

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epub
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keywords
Journal Article
in
Nanomedicine: Nanotechnology, Biology, and Medicine
publisher
Elsevier
external identifiers
  • scopus:85017656573
  • wos:000402678800022
ISSN
1549-9642
DOI
10.1016/j.nano.2016.12.023
language
English
LU publication?
yes
id
4a32c0c4-04b4-40d3-9cf3-13fe728b69a5
date added to LUP
2017-03-13 13:17:24
date last changed
2017-09-18 11:38:59
@article{4a32c0c4-04b4-40d3-9cf3-13fe728b69a5,
  abstract     = {<p>We demonstrate an artificial three-dimensional (3D) electrical active human neuronal network system, by the growth of brain neural progenitors in highly porous low density electrospun poly-ε-caprolactone (PCL) fiber scaffolds. In neuroscience research cell-based assays are important experimental instruments for studying neuronal function in health and disease. Traditional cell culture at 2D-surfaces induces abnormal cell-cell contacts and network formation. Hence, there is a tremendous need to explore in vivo-resembling 3D neural cell culture approaches. We present an improved electrospinning method for fabrication of scaffolds that promote neuronal differentiation into highly 3D integrated networks, formation of inhibitory and excitatory synapses and extensive neurite growth. Notably, in 3D scaffolds in vivo-resembling intermixed neuronal and glial cell network were formed, whereas in parallel 2D cultures a neuronal cell layer grew separated from an underlying glial cell layer. Hence, the use of the 3D cell assay presented will most likely provide more physiological relevant results.</p>},
  author       = {Jakobsson, Albin and Ottosson, Maximilian and Zalis, Marina Castro and O'Carroll, David and Johansson, Ulrica Englund and Johansson, Per Fredrik},
  issn         = {1549-9642},
  keyword      = {Journal Article},
  language     = {eng},
  month        = {01},
  publisher    = {Elsevier},
  series       = {Nanomedicine: Nanotechnology, Biology, and Medicine},
  title        = {Three-dimensional functional human neuronal networks in uncompressed low-density electrospun fiber scaffolds},
  url          = {http://dx.doi.org/10.1016/j.nano.2016.12.023},
  year         = {2017},
}