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Anti-Fibrotic Activity of an Antimicrobial Peptide in a Drosophila Model

Khalili, Dilan ; Kalcher, Christina ; Baumgartner, Stefan LU orcid and Theopold, Ulrich (2021) In Journal of Innate Immunity 13(6). p.376-390
Abstract

Fibrotic lesions accompany several pathological conditions, including tumors. We show that expression of a dominant-active form of the Ras oncogene in Drosophila salivary glands (SGs) leads to redistribution of components of the basement membrane (BM) and fibrotic lesions. Similar to several types of mammalian fibrosis, the disturbed BM attracts clot components, including insect transglutaminase and phenoloxidase. SG epithelial cells show reduced apicobasal polarity accompanied by a loss of secretory activity. Both the fibrotic lesions and the reduced cell polarity are alleviated by ectopic expression of the antimicrobial peptide drosomycin (Drs), which also restores the secretory activity of the SGs. In addition to extracellular matrix... (More)

Fibrotic lesions accompany several pathological conditions, including tumors. We show that expression of a dominant-active form of the Ras oncogene in Drosophila salivary glands (SGs) leads to redistribution of components of the basement membrane (BM) and fibrotic lesions. Similar to several types of mammalian fibrosis, the disturbed BM attracts clot components, including insect transglutaminase and phenoloxidase. SG epithelial cells show reduced apicobasal polarity accompanied by a loss of secretory activity. Both the fibrotic lesions and the reduced cell polarity are alleviated by ectopic expression of the antimicrobial peptide drosomycin (Drs), which also restores the secretory activity of the SGs. In addition to extracellular matrix components, both Drs and F-actin localize to fibrotic lesions.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antimicrobial peptides, Extracellular matrix, Fibrosis, Innate immunity, Insect immunity
in
Journal of Innate Immunity
volume
13
issue
6
pages
376 - 390
publisher
Karger
external identifiers
  • scopus:85107491293
  • pmid:34000729
ISSN
1662-811X
DOI
10.1159/000516104
language
English
LU publication?
yes
id
4aaade5d-9fc7-4476-8031-98d563c40353
date added to LUP
2021-07-09 14:11:09
date last changed
2024-06-15 13:20:45
@article{4aaade5d-9fc7-4476-8031-98d563c40353,
  abstract     = {{<p>Fibrotic lesions accompany several pathological conditions, including tumors. We show that expression of a dominant-active form of the Ras oncogene in Drosophila salivary glands (SGs) leads to redistribution of components of the basement membrane (BM) and fibrotic lesions. Similar to several types of mammalian fibrosis, the disturbed BM attracts clot components, including insect transglutaminase and phenoloxidase. SG epithelial cells show reduced apicobasal polarity accompanied by a loss of secretory activity. Both the fibrotic lesions and the reduced cell polarity are alleviated by ectopic expression of the antimicrobial peptide drosomycin (Drs), which also restores the secretory activity of the SGs. In addition to extracellular matrix components, both Drs and F-actin localize to fibrotic lesions. </p>}},
  author       = {{Khalili, Dilan and Kalcher, Christina and Baumgartner, Stefan and Theopold, Ulrich}},
  issn         = {{1662-811X}},
  keywords     = {{Antimicrobial peptides; Extracellular matrix; Fibrosis; Innate immunity; Insect immunity}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{376--390}},
  publisher    = {{Karger}},
  series       = {{Journal of Innate Immunity}},
  title        = {{Anti-Fibrotic Activity of an Antimicrobial Peptide in a Drosophila Model}},
  url          = {{http://dx.doi.org/10.1159/000516104}},
  doi          = {{10.1159/000516104}},
  volume       = {{13}},
  year         = {{2021}},
}