Amyloid-β oligomers are inefficiently measured by enzyme-linked immunosorbent assay
Stenh, Charlotte ; Englund, Hillevi ; Lord, Anna ; Johansson, Ann Sofi ; Almeida, Claudia G. ; Gellerfors, Pär ; Greengard, Paul ; Gouras, Gunnar K. LU
; Lannfelt, Lars
and Nilsson, Lars N.G.
(2005)
In Annals of Neurology
58(1).
p.147-150
- Abstract
Amyloid-β (Aβ) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Aβ results in underestimated Aβ ELISA levels. The implications are that comprehensive analysis of soluble Aβ requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4ab63dba-d5a6-4370-ad22-25873d3434d8
- author
- Stenh, Charlotte
; Englund, Hillevi
; Lord, Anna
; Johansson, Ann Sofi
; Almeida, Claudia G.
; Gellerfors, Pär
; Greengard, Paul
; Gouras, Gunnar K.
LU
; Lannfelt, Lars
and Nilsson, Lars N.G.
- publishing date
- 2005-07
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Annals of Neurology
- volume
- 58
- issue
- 1
- pages
- 147 - 150
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:15984012
- scopus:21844448609
- ISSN
- 0364-5134
- DOI
- 10.1002/ana.20524
- language
- English
- LU publication?
- no
- id
- 4ab63dba-d5a6-4370-ad22-25873d3434d8
- date added to LUP
- 2020-02-20 14:32:03
- date last changed
- 2024-04-03 01:32:55
@article{4ab63dba-d5a6-4370-ad22-25873d3434d8,
abstract = {{<p>Amyloid-β (Aβ) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Aβ results in underestimated Aβ ELISA levels. The implications are that comprehensive analysis of soluble Aβ requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.</p>}},
author = {{Stenh, Charlotte and Englund, Hillevi and Lord, Anna and Johansson, Ann Sofi and Almeida, Claudia G. and Gellerfors, Pär and Greengard, Paul and Gouras, Gunnar K. and Lannfelt, Lars and Nilsson, Lars N.G.}},
issn = {{0364-5134}},
language = {{eng}},
number = {{1}},
pages = {{147--150}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Annals of Neurology}},
title = {{Amyloid-β oligomers are inefficiently measured by enzyme-linked immunosorbent assay}},
url = {{http://dx.doi.org/10.1002/ana.20524}},
doi = {{10.1002/ana.20524}},
volume = {{58}},
year = {{2005}},
}