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Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

Ghatak, Souvik LU ; Lallawmzuali, Doris ; Mukherjee, Subhajit ; Mawia, Lal ; Pautu, Jeremy L and Kumar, Nachimuthu Senthil (2016) In Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis 27(3). p.8-2205
Abstract

Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer... (More)

Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarkers, Tumor/genetics, Breast Neoplasms/blood, DNA, Mitochondrial/genetics, Female, Genes, Mitochondrial, Genome/genetics, Genome, Mitochondrial/genetics, Humans, Mitochondria/genetics, Mongolia, Polymerase Chain Reaction, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length/genetics, Sequence Analysis, DNA/methods, Whole Genome Sequencing/methods
in
Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis
volume
27
issue
3
pages
8 - 2205
publisher
Taylor & Francis
external identifiers
  • pmid:25431825
  • scopus:85040700879
ISSN
2470-1394
DOI
10.3109/19401736.2014.982627
language
English
LU publication?
no
id
4b50e761-8f37-4e7d-a97f-98760fa2c9f8
date added to LUP
2021-11-10 09:52:29
date last changed
2024-10-06 08:59:35
@article{4b50e761-8f37-4e7d-a97f-98760fa2c9f8,
  abstract     = {{<p>Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.</p>}},
  author       = {{Ghatak, Souvik and Lallawmzuali, Doris and Mukherjee, Subhajit and Mawia, Lal and Pautu, Jeremy L and Kumar, Nachimuthu Senthil}},
  issn         = {{2470-1394}},
  keywords     = {{Biomarkers, Tumor/genetics; Breast Neoplasms/blood; DNA, Mitochondrial/genetics; Female; Genes, Mitochondrial; Genome/genetics; Genome, Mitochondrial/genetics; Humans; Mitochondria/genetics; Mongolia; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length/genetics; Sequence Analysis, DNA/methods; Whole Genome Sequencing/methods}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{8--2205}},
  publisher    = {{Taylor & Francis}},
  series       = {{Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis}},
  title        = {{Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis}},
  url          = {{http://dx.doi.org/10.3109/19401736.2014.982627}},
  doi          = {{10.3109/19401736.2014.982627}},
  volume       = {{27}},
  year         = {{2016}},
}