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γ-Glutamyltransferase bound to prostatic subcellular organelles and in free form in human seminal plasma

Lilja, H. LU orcid and Weiber, H. LU (1983) In Scandinavian Journal of Clinical and Laboratory Investigation 43(4). p.307-312
Abstract

Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg+plus;-Ca+plus;-dependent ATPase, and Zn+plus;-dependent peptidase hydrolysing succinyl (alanine)3-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn+plus;-dependent peptidase activity, nor of the Mg+plus;-Ca+plus; dependent... (More)

Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg+plus;-Ca+plus;-dependent ATPase, and Zn+plus;-dependent peptidase hydrolysing succinyl (alanine)3-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn+plus;-dependent peptidase activity, nor of the Mg+plus;-Ca+plus; dependent ATPase activity, both having been inactivated by the papain. Both organelle-bound γglutamyltransferase and Zn+plus;-dependent peptidase could be rendered soluble in active form with the non-ionic detergent Triton X-100.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adenosine triphosphatase, Membrane-bound proteins, Peptidohydrolase, Prostate, Semen, γglutamyl transpeptidase
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
43
issue
4
pages
307 - 312
publisher
Informa Healthcare
external identifiers
  • pmid:6138849
  • scopus:0020537468
ISSN
0036-5513
DOI
10.1080/00365518309168262
language
English
LU publication?
yes
id
4ba7a43b-cb1b-4a51-a304-867b832674b5
date added to LUP
2022-12-06 16:55:09
date last changed
2024-01-03 19:31:05
@article{4ba7a43b-cb1b-4a51-a304-867b832674b5,
  abstract     = {{<p>Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg<sup>+plus;</sup>-Ca<sup>+plus;</sup>-dependent ATPase, and Zn<sup>+plus;</sup>-dependent peptidase hydrolysing succinyl (alanine)<sub>3</sub>-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn<sup>+plus;</sup>-dependent peptidase activity, nor of the Mg<sup>+plus;</sup>-Ca<sup>+plus;</sup> dependent ATPase activity, both having been inactivated by the papain. Both organelle-bound γglutamyltransferase and Zn<sup>+plus;</sup>-dependent peptidase could be rendered soluble in active form with the non-ionic detergent Triton X-100.</p>}},
  author       = {{Lilja, H. and Weiber, H.}},
  issn         = {{0036-5513}},
  keywords     = {{Adenosine triphosphatase; Membrane-bound proteins; Peptidohydrolase; Prostate; Semen; γglutamyl transpeptidase}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{307--312}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{γ-Glutamyltransferase bound to prostatic subcellular organelles and in free form in human seminal plasma}},
  url          = {{http://dx.doi.org/10.1080/00365518309168262}},
  doi          = {{10.1080/00365518309168262}},
  volume       = {{43}},
  year         = {{1983}},
}