γ-Glutamyltransferase bound to prostatic subcellular organelles and in free form in human seminal plasma
(1983) In Scandinavian Journal of Clinical and Laboratory Investigation 43(4). p.307-312- Abstract
Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg+plus;-Ca+plus;-dependent ATPase, and Zn+plus;-dependent peptidase hydrolysing succinyl (alanine)3-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn+plus;-dependent peptidase activity, nor of the Mg+plus;-Ca+plus; dependent... (More)
Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg+plus;-Ca+plus;-dependent ATPase, and Zn+plus;-dependent peptidase hydrolysing succinyl (alanine)3-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn+plus;-dependent peptidase activity, nor of the Mg+plus;-Ca+plus; dependent ATPase activity, both having been inactivated by the papain. Both organelle-bound γglutamyltransferase and Zn+plus;-dependent peptidase could be rendered soluble in active form with the non-ionic detergent Triton X-100.
(Less)
- author
- Lilja, H. LU and Weiber, H. LU
- organization
- publishing date
- 1983
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adenosine triphosphatase, Membrane-bound proteins, Peptidohydrolase, Prostate, Semen, γglutamyl transpeptidase
- in
- Scandinavian Journal of Clinical and Laboratory Investigation
- volume
- 43
- issue
- 4
- pages
- 307 - 312
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:6138849
- scopus:0020537468
- ISSN
- 0036-5513
- DOI
- 10.1080/00365518309168262
- language
- English
- LU publication?
- yes
- id
- 4ba7a43b-cb1b-4a51-a304-867b832674b5
- date added to LUP
- 2022-12-06 16:55:09
- date last changed
- 2024-01-03 19:31:05
@article{4ba7a43b-cb1b-4a51-a304-867b832674b5, abstract = {{<p>Most of the γglutamyltransferase occurring in semen was found to be bound to the membranes of prostatic organelles, as is the case with Mg<sup>+plus;</sup>-Ca<sup>+plus;</sup>-dependent ATPase, and Zn<sup>+plus;</sup>-dependent peptidase hydrolysing succinyl (alanine)<sub>3</sub>-paranitroanilide. Organelie-bound γglutamyltransferase was released in a water-soluble form by papain. Charge shift electrophoresis revealed the presence in seminal fluid of a small additional amount of γglutamyltransferase that is unbound and water-soluble, but also of prostatic origin. After papain treatment there was no sign of the organelle-bound Zn<sup>+plus;</sup>-dependent peptidase activity, nor of the Mg<sup>+plus;</sup>-Ca<sup>+plus;</sup> dependent ATPase activity, both having been inactivated by the papain. Both organelle-bound γglutamyltransferase and Zn<sup>+plus;</sup>-dependent peptidase could be rendered soluble in active form with the non-ionic detergent Triton X-100.</p>}}, author = {{Lilja, H. and Weiber, H.}}, issn = {{0036-5513}}, keywords = {{Adenosine triphosphatase; Membrane-bound proteins; Peptidohydrolase; Prostate; Semen; γglutamyl transpeptidase}}, language = {{eng}}, number = {{4}}, pages = {{307--312}}, publisher = {{Informa Healthcare}}, series = {{Scandinavian Journal of Clinical and Laboratory Investigation}}, title = {{γ-Glutamyltransferase bound to prostatic subcellular organelles and in free form in human seminal plasma}}, url = {{http://dx.doi.org/10.1080/00365518309168262}}, doi = {{10.1080/00365518309168262}}, volume = {{43}}, year = {{1983}}, }