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Progressive changes in human follicular fluid composition over the course of ovulation : quantitative proteomic analyses

Poulsen, Liv la Cour; Pla, Indira LU ; Sanchez, A. LU ; Grøndahl, Marie Louise; Marko-Varga, György LU ; Yding Andersen, C.; Englund, Anne Lis Mikkelsen and Malm, J. LU (2019) In Molecular and Cellular Endocrinology 495(Sept.).
Abstract

Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12–17 h, e.g., AREG... (More)

Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12–17 h, e.g., AREG (p < 0.0001), TNFAIP6 (p < 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p < 0.0001), TIMP1 (p < 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Follicular fluid, Human, Oocyte maturation, Ovulation, Proteome
in
Molecular and Cellular Endocrinology
volume
495
issue
Sept.
publisher
Elsevier
external identifiers
  • scopus:85069970415
ISSN
0303-7207
DOI
10.1016/j.mce.2019.110522
language
English
LU publication?
yes
id
4bb9b37c-4040-463f-ab7a-f0b2bfed2602
date added to LUP
2019-08-30 09:57:40
date last changed
2019-09-04 04:49:38
@article{4bb9b37c-4040-463f-ab7a-f0b2bfed2602,
  abstract     = {<p>Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p &lt; 0.05) and on-off expression patterns. The majority peaked after 12–17 h, e.g., AREG (p &lt; 0.0001), TNFAIP6 (p &lt; 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p &lt; 0.0001), TIMP1 (p &lt; 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.</p>},
  articleno    = {110522},
  author       = {Poulsen, Liv la Cour and Pla, Indira and Sanchez, A. and Grøndahl, Marie Louise and Marko-Varga, György and Yding Andersen, C. and Englund, Anne Lis Mikkelsen and Malm, J.},
  issn         = {0303-7207},
  keyword      = {Follicular fluid,Human,Oocyte maturation,Ovulation,Proteome},
  language     = {eng},
  number       = {Sept.},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {Progressive changes in human follicular fluid composition over the course of ovulation : quantitative proteomic analyses},
  url          = {http://dx.doi.org/10.1016/j.mce.2019.110522},
  volume       = {495},
  year         = {2019},
}