Assignment of the low-temperature fluorescence in oxygen-evolving Photosystem II
(2005) In Photosynthesis Research 84(1-3). p.193-199- Abstract
- Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in... (More)
- Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in CP43 or CP47 is located within its inhomogeneous distribution. PS II fluorescence arises from CP43 and CP47 'slow-transfer' states, convolved by this dependence. At higher temperatures, thermally activated excitation transfer to the PS II charge-separating system bypasses such bottlenecks. As the charge-separating state of active PS II absorbs at >700 nm, PS II emission in the 680-700 nm region is unlikely to arise from reaction centre pigments. PS II emission at physiological temperatures is discussed in terms of these results. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/231414
- author
- Krausz, E ; Hughes, J L ; Smith, P J ; Pace, R J and Peterson Årsköld, Sindra LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- fluorescence, fluorescence line narrowing, energy transfer, PSII
- in
- Photosynthesis Research
- volume
- 84
- issue
- 1-3
- pages
- 193 - 199
- publisher
- Springer
- external identifiers
-
- wos:000230845200029
- pmid:16049774
- scopus:23444436729
- pmid:16049774
- ISSN
- 0166-8595
- DOI
- 10.1007/s11120-004-7078-9
- language
- English
- LU publication?
- yes
- id
- 4bf488a6-aee8-4a3e-8458-b90ec4441729 (old id 231414)
- date added to LUP
- 2016-04-01 16:38:05
- date last changed
- 2022-01-28 21:01:25
@article{4bf488a6-aee8-4a3e-8458-b90ec4441729,
abstract = {{Low-temperature absorption and fluorescence spectra of fully active cores and membrane-bound PS II preparations are compared. Detailed temperature dependence of fluorescence spectra between 5 and 70 K are presented as well as 1.7-K fluorescence line-narrowed (FLN) spectra of cores, confirming that PS II emission is composite. Spectra are compared to those reported for LHCII, CP43, CP47 and D1/D2/cytb(559) subunits of PS II. A combination of subunit spectra cannot account for emission of active PS II. The complex temperature dependence of PS II fluorescence is interpretable by noting that excitation transfer from CP43 and CP47 to the reaction centre is slow, and strongly dependent on the precise energy at which a 'slow-transfer' pigment in CP43 or CP47 is located within its inhomogeneous distribution. PS II fluorescence arises from CP43 and CP47 'slow-transfer' states, convolved by this dependence. At higher temperatures, thermally activated excitation transfer to the PS II charge-separating system bypasses such bottlenecks. As the charge-separating state of active PS II absorbs at >700 nm, PS II emission in the 680-700 nm region is unlikely to arise from reaction centre pigments. PS II emission at physiological temperatures is discussed in terms of these results.}},
author = {{Krausz, E and Hughes, J L and Smith, P J and Pace, R J and Peterson Årsköld, Sindra}},
issn = {{0166-8595}},
keywords = {{fluorescence; fluorescence line narrowing; energy transfer; PSII}},
language = {{eng}},
number = {{1-3}},
pages = {{193--199}},
publisher = {{Springer}},
series = {{Photosynthesis Research}},
title = {{Assignment of the low-temperature fluorescence in oxygen-evolving Photosystem II}},
url = {{http://dx.doi.org/10.1007/s11120-004-7078-9}},
doi = {{10.1007/s11120-004-7078-9}},
volume = {{84}},
year = {{2005}},
}