From in situ to ex vivo: the effect of autolysis and fixation on quantitative MRI markers for myelin
Mohammadi, Siawoosh ; Sedlacik, Jan ; Callaghan, Martina F ; Fieler, Jens ; Helms, Gunther LU
and Sprenger, Christian
(2017)
ISMRM 25th Annual Meeting and Exhibition
In Proc Intl Soc Magn Reson Med
25.
- Abstract
- Ex vivo histology remains the gold standard against which MRI biophysical models, e.g. the MR g-ratio which characterises the fraction of a fibre’s diameter that is myelinated, are evaluated. The MR g-ratio model requires a measure of myelin density, for which magnetization transfer saturation (MT) has been used as a biomarker. However, changes occurring post mortem, e.g. autolysis, temperature changes and fixation, significantly alter the MRI signal. Here we investigate how these changes impact MT. We found that MT decreased post mortem but greatly
increased upon fixation. These effects are similar to reported changes of other established MRI myelin-markers.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4c9de42b-ef0d-4d4f-a250-d77569a80222
- author
- Mohammadi, Siawoosh
; Sedlacik, Jan
; Callaghan, Martina F
; Fieler, Jens
; Helms, Gunther
LU
and Sprenger, Christian
- organization
- publishing date
- 2017-04-07
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- ISMRM 25th Annual Meeting Proceedings
- series title
- Proc Intl Soc Magn Reson Med
- volume
- 25
- article number
- 3764
- conference name
- ISMRM 25th Annual Meeting and Exhibition
- conference location
- Honolulu, United States
- conference dates
- 2017-04-22 - 2017-04-27
- ISSN
- 1545-4428
- language
- English
- LU publication?
- yes
- id
- 4c9de42b-ef0d-4d4f-a250-d77569a80222
- alternative location
- https://cds.ismrm.org/protected/17MProceedings/PDFfiles/3764.html
- date added to LUP
- 2021-05-18 21:57:37
- date last changed
- 2022-03-16 13:53:09
@inproceedings{4c9de42b-ef0d-4d4f-a250-d77569a80222,
abstract = {{<i>Ex vivo</i> histology remains the gold standard against which MRI biophysical models, e.g. the MR g-ratio which characterises the fraction of a fibre’s diameter that is myelinated, are evaluated. The MR g-ratio model requires a measure of myelin density, for which magnetization transfer saturation (MT) has been used as a biomarker. However, changes occurring post mortem, e.g. autolysis, temperature changes and fixation, significantly alter the MRI signal. Here we investigate how these changes impact MT. We found that MT decreased post mortem but greatly<br/>increased upon fixation. These effects are similar to reported changes of other established MRI myelin-markers.}},
author = {{Mohammadi, Siawoosh and Sedlacik, Jan and Callaghan, Martina F and Fieler, Jens and Helms, Gunther and Sprenger, Christian}},
booktitle = {{ISMRM 25th Annual Meeting Proceedings}},
issn = {{1545-4428}},
language = {{eng}},
month = {{04}},
series = {{Proc Intl Soc Magn Reson Med}},
title = {{From in situ to ex vivo: the effect of autolysis and fixation on quantitative MRI markers for myelin}},
url = {{https://cds.ismrm.org/protected/17MProceedings/PDFfiles/3764.html}},
volume = {{25}},
year = {{2017}},
}