Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.
(2004) In Physiological Genomics 19(2). p.228-232- Abstract
- A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic... (More)
- A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene. (Less)
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https://lup.lub.lu.se/record/1130253
- author
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physiological Genomics
- volume
- 19
- issue
- 2
- pages
- 228 - 232
- publisher
- American Physiological Society
- external identifiers
-
- scopus:19944386204
- pmid:15328390
- ISSN
- 1094-8341
- DOI
- 10.1152/physiolgenomics.00126.2004
- language
- English
- LU publication?
- no
- id
- 4ca489e9-eb11-4204-8748-7520ddab92be (old id 1130253)
- date added to LUP
- 2016-04-01 11:35:02
- date last changed
- 2022-01-26 07:15:11
@article{4ca489e9-eb11-4204-8748-7520ddab92be, abstract = {{A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.}}, author = {{Michalkiewicz, Mieczyslaw and Michalkiewicz, Teresa and Ettinger, Ruth A. and Rutledge, Elizabeth A. and Fuller, Jessica M. and Moralejo, Daniel H. and Van Yserloo, Brian and MacMurray, Armand J. and Kwitek, Anne E. and Jacob, Howard J. and Lander, Eric S. and Lernmark, Åke}}, issn = {{1094-8341}}, language = {{eng}}, number = {{2}}, pages = {{228--232}}, publisher = {{American Physiological Society}}, series = {{Physiological Genomics}}, title = {{Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.}}, url = {{http://dx.doi.org/10.1152/physiolgenomics.00126.2004}}, doi = {{10.1152/physiolgenomics.00126.2004}}, volume = {{19}}, year = {{2004}}, }