Induction of Vascular Remodeling in the Lung by Chronic House Dust Mite Exposure.
Rydell-Törmänen, Kristina LU
; Johnson, Jill R
; Fattouh, Ramzi
; Jordana, Manel
and Erjefält, Jonas
LU
(2008)
In American Journal of Respiratory Cell and Molecular Biology
39.
p.61-67
- Abstract
- Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were... (More)
- Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were exposed to HDM intranasally for 7 or 20 consecutive weeks, and resolution of the inflammatory and remodeling response to allergen was investigated 4 weeks following the end of a 7-week exposure protocol. Measures of vascular remodeling, including total collagen deposition, procollagen I-production, endothelial and smooth muscle cell proliferation, smooth muscle area and presence of myofibroblasts were investigated histologically in lung vessels of different sizes and locations. We observed an increase in total collagen content which did not resolve upon cessation of allergen exposure. Other parameters were significantly increased following 7 and/or 20 weeks of allergen exposure, but returned to baseline following allergen withdrawal. We conclude that respiratory HDM exposure induces not only airway remodeling, but also pulmonary vascular remodeling, and in accordance with airway remodeling, some components of these structural changes may be irreversible. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052861
- author
- Rydell-Törmänen, Kristina
LU
; Johnson, Jill R
; Fattouh, Ramzi
; Jordana, Manel
and Erjefält, Jonas
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Respiratory Cell and Molecular Biology
- volume
- 39
- pages
- 61 - 67
- publisher
- American Thoracic Society
- external identifiers
-
- pmid:18314535
- wos:000257037600008
- scopus:45849114330
- pmid:18314535
- ISSN
- 1535-4989
- DOI
- 10.1165/rcmb.2007-0441OC
- language
- English
- LU publication?
- yes
- id
- 4cacf7c1-ebc7-4b2b-ada3-8b8641dc3a06 (old id 1052861)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18314535?dopt=Abstract
- date added to LUP
- 2016-04-04 08:28:11
- date last changed
- 2022-03-23 02:20:45
@article{4cacf7c1-ebc7-4b2b-ada3-8b8641dc3a06,
abstract = {{Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were exposed to HDM intranasally for 7 or 20 consecutive weeks, and resolution of the inflammatory and remodeling response to allergen was investigated 4 weeks following the end of a 7-week exposure protocol. Measures of vascular remodeling, including total collagen deposition, procollagen I-production, endothelial and smooth muscle cell proliferation, smooth muscle area and presence of myofibroblasts were investigated histologically in lung vessels of different sizes and locations. We observed an increase in total collagen content which did not resolve upon cessation of allergen exposure. Other parameters were significantly increased following 7 and/or 20 weeks of allergen exposure, but returned to baseline following allergen withdrawal. We conclude that respiratory HDM exposure induces not only airway remodeling, but also pulmonary vascular remodeling, and in accordance with airway remodeling, some components of these structural changes may be irreversible.}},
author = {{Rydell-Törmänen, Kristina and Johnson, Jill R and Fattouh, Ramzi and Jordana, Manel and Erjefält, Jonas}},
issn = {{1535-4989}},
language = {{eng}},
pages = {{61--67}},
publisher = {{American Thoracic Society}},
series = {{American Journal of Respiratory Cell and Molecular Biology}},
title = {{Induction of Vascular Remodeling in the Lung by Chronic House Dust Mite Exposure.}},
url = {{http://dx.doi.org/10.1165/rcmb.2007-0441OC}},
doi = {{10.1165/rcmb.2007-0441OC}},
volume = {{39}},
year = {{2008}},
}