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Four-dimensional in vivo X-ray microscopy with projection-guided gating

Mokso, Rajmund LU ; Schwyn, Daniel A.; Walker, Simon M.; Doube, Michael; Wicklein, Martina; Müller, Tonya; Stampanoni, Marco; Taylor, Graham K. and Krapp, Holger G. (2015) In Scientific Reports 5.
Abstract

Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.

Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
in
Scientific Reports
volume
5
publisher
Nature Publishing Group
external identifiers
  • scopus:84924869329
ISSN
2045-2322
DOI
10.1038/srep08727
language
English
LU publication?
no
id
4cf28b13-866a-48aa-8bcc-31ac25b22a65
date added to LUP
2017-09-19 14:38:06
date last changed
2017-11-12 04:35:15
@article{4cf28b13-866a-48aa-8bcc-31ac25b22a65,
  abstract     = {<p>Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.</p>},
  articleno    = {8727},
  author       = {Mokso, Rajmund and Schwyn, Daniel A. and Walker, Simon M. and Doube, Michael and Wicklein, Martina and Müller, Tonya and Stampanoni, Marco and Taylor, Graham K. and Krapp, Holger G.},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Four-dimensional in vivo X-ray microscopy with projection-guided gating},
  url          = {http://dx.doi.org/10.1038/srep08727},
  volume       = {5},
  year         = {2015},
}