Acoustofluidic rare cell sample preparation
(2015)- Abstract
- Acoustofluidics utilizes a combination of acoustics, in the form of ultrasound, and microfluidics to manipulate cells
and particles. This has proven to be a versatile method that is gentle to the cells. In this thesis acoustofluidics has been used for
processing rare cells in continuous flow. Rare cells are within this thesis defined as cells that are present in numbers of 1-1000
per mL in a much larger population of background cells. Rare cells present in blood have been of particular interest, and cancer
cells and bacteria have been used as model cells. In this thesis acoustofluidics has first been used to concentrate cells. This was
done by using two-dimensional focusing and a multistage... (More) - Acoustofluidics utilizes a combination of acoustics, in the form of ultrasound, and microfluidics to manipulate cells
and particles. This has proven to be a versatile method that is gentle to the cells. In this thesis acoustofluidics has been used for
processing rare cells in continuous flow. Rare cells are within this thesis defined as cells that are present in numbers of 1-1000
per mL in a much larger population of background cells. Rare cells present in blood have been of particular interest, and cancer
cells and bacteria have been used as model cells. In this thesis acoustofluidics has first been used to concentrate cells. This was
done by using two-dimensional focusing and a multistage acoustofluidic device where sequential concentration steps,
generating moderate concentration factors, could be multiplied into large concentration factors. The usefulness of the method
was then extended as the critical particle focusing size was lowered to also allow focusing of bacteria. This was done through
using two-dimensional focusing, which was shown to change the acoustic streaming pattern to no longer counteract the primary
acoustic radiation force. The new critical particle focusing size was determined to be between 0.5 μm and 0.24 μm in particle
diameter for polystyrene-like particles. In the third paper a simplyfied acoustofluidic device, that does not rely on a clean fluid
sheath flow to prealign the cells or particles before the separation, was presented. To be able to do this the device used only
two-dimensional focusing to prealign the cells. The usefulness of the device was in turn demonstrated with the separation of
cancer cells from white blood cells where it was shown to perform comparably to previously presented devices. In the fourth
paper a separation method was combined with the concentration method presented in the first paper on an integrated device.
The device was shown to be able to simultaneously separate and concentrate cancer cells from white blood cells. Finally, the
previously proposed concentration device was integrated with a DEP single cell trapping device further showing the usefulness
of the acoustofluidic method. Standing alone, the DEP trapping device could only process sample at a flow rate of 4 μL/min
while still maintaining a high trapping efficiency.By integrating the DEP trapping device with the acoustofluidic concentrator
device a higher sample inflow rate could be used as the acoustofluidic device could gear down the flow rate before the sample
entered the DEP trapping device. Together samples could be processed ~10 times faster than using the DEP trapping device
alone, while still recovering over 90% of the cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8171879
- author
- Antfolk, Maria LU
- supervisor
- opponent
-
- Lee, Abraham, University of California, Irvine
- organization
- publishing date
- 2015
- type
- Thesis
- publication status
- published
- subject
- defense location
- Lecture hall 1406, building E, Ole Römers väg 3, Lund University, Faculty of Engineering LTH, Lund
- defense date
- 2015-12-11 09:15:00
- ISBN
- 978-91-7623-527-0
- language
- English
- LU publication?
- yes
- id
- 4d093d06-f5a8-4b67-ad1c-3e0cd1db40df (old id 8171879)
- date added to LUP
- 2016-04-04 13:20:11
- date last changed
- 2019-11-04 11:08:38
@phdthesis{4d093d06-f5a8-4b67-ad1c-3e0cd1db40df,
abstract = {{Acoustofluidics utilizes a combination of acoustics, in the form of ultrasound, and microfluidics to manipulate cells<br/><br>
and particles. This has proven to be a versatile method that is gentle to the cells. In this thesis acoustofluidics has been used for<br/><br>
processing rare cells in continuous flow. Rare cells are within this thesis defined as cells that are present in numbers of 1-1000<br/><br>
per mL in a much larger population of background cells. Rare cells present in blood have been of particular interest, and cancer<br/><br>
cells and bacteria have been used as model cells. In this thesis acoustofluidics has first been used to concentrate cells. This was<br/><br>
done by using two-dimensional focusing and a multistage acoustofluidic device where sequential concentration steps,<br/><br>
generating moderate concentration factors, could be multiplied into large concentration factors. The usefulness of the method<br/><br>
was then extended as the critical particle focusing size was lowered to also allow focusing of bacteria. This was done through<br/><br>
using two-dimensional focusing, which was shown to change the acoustic streaming pattern to no longer counteract the primary<br/><br>
acoustic radiation force. The new critical particle focusing size was determined to be between 0.5 μm and 0.24 μm in particle<br/><br>
diameter for polystyrene-like particles. In the third paper a simplyfied acoustofluidic device, that does not rely on a clean fluid<br/><br>
sheath flow to prealign the cells or particles before the separation, was presented. To be able to do this the device used only<br/><br>
two-dimensional focusing to prealign the cells. The usefulness of the device was in turn demonstrated with the separation of<br/><br>
cancer cells from white blood cells where it was shown to perform comparably to previously presented devices. In the fourth<br/><br>
paper a separation method was combined with the concentration method presented in the first paper on an integrated device.<br/><br>
The device was shown to be able to simultaneously separate and concentrate cancer cells from white blood cells. Finally, the<br/><br>
previously proposed concentration device was integrated with a DEP single cell trapping device further showing the usefulness<br/><br>
of the acoustofluidic method. Standing alone, the DEP trapping device could only process sample at a flow rate of 4 μL/min<br/><br>
while still maintaining a high trapping efficiency.By integrating the DEP trapping device with the acoustofluidic concentrator<br/><br>
device a higher sample inflow rate could be used as the acoustofluidic device could gear down the flow rate before the sample<br/><br>
entered the DEP trapping device. Together samples could be processed ~10 times faster than using the DEP trapping device<br/><br>
alone, while still recovering over 90% of the cells.}},
author = {{Antfolk, Maria}},
isbn = {{978-91-7623-527-0}},
language = {{eng}},
school = {{Lund University}},
title = {{Acoustofluidic rare cell sample preparation}},
url = {{https://lup.lub.lu.se/search/files/6094988/8171880.pdf}},
year = {{2015}},
}