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Metabolomics of transgenic maize combining Fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extraction

Leon, Emmanuel Carlos Dean; Rodriguez-Meizoso, Irene LU ; Lucio, Marianna; Garcia-Cañas, Virginia; Ibañez, Elena; Schmitt-Kopplin, Philippe and Cifuentes, Alejandro (2009) In Journal of Chromatography A 1216(43). p.7314-7323
Abstract

In this work, the potential of combining capillary electrophoresis-time-of-flight-mass spectrome-try (CE-TOF-MS) and Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) for metabolomics of genetically modified organisms (GMOs) is demonstrated. Thus, six different varieties of maize, three of them transgenic (PR33P66 Bt, Tietar Bt and Aristis Bt) and their corresponding isogenic lines (PR33P66, Tietar and Aristis) grown under the same field conditions, were analyzed. Based on the ultrahigh resolution and remarkable mass accuracy provided by the 12-T FT-ICR-MS it was possible to directly analyze a good number of metabolites whose identity could be proposed based on their specific isotopic pattern. For identification of... (More)

In this work, the potential of combining capillary electrophoresis-time-of-flight-mass spectrome-try (CE-TOF-MS) and Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) for metabolomics of genetically modified organisms (GMOs) is demonstrated. Thus, six different varieties of maize, three of them transgenic (PR33P66 Bt, Tietar Bt and Aristis Bt) and their corresponding isogenic lines (PR33P66, Tietar and Aristis) grown under the same field conditions, were analyzed. Based on the ultrahigh resolution and remarkable mass accuracy provided by the 12-T FT-ICR-MS it was possible to directly analyze a good number of metabolites whose identity could be proposed based on their specific isotopic pattern. For identification of metabolite isomers, CE-TOF-MS was also used combining the information on nominal mass with electrophoretic mobility corroborating in that way the identity of several new biomarkers. Furthermore, PLE extractions were evaluated in order to establish selective extraction as an additional criterion to obtain useful information in maize metabolomics. Differences in the metabolite levels were found between the three transgenic maize varieties compared with their wild isogenic lines in some specific metabolic pathways. To our knowledge, this is the first time that an approach as the one presented in this work (pressurized liquid extraction + FT-ICR-MS + CE-TOF-MS) is shown for a metabolomic study.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Bt maize, Capillary electrophoresis-mass spectrometry (CE-MS), Corn, FT-MS, GMOs, Metabolites, Metabolomics, Transgenic foods
in
Journal of Chromatography A
volume
1216
issue
43
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:74049156423
ISSN
0021-9673
DOI
10.1016/j.chroma.2009.04.092
language
English
LU publication?
no
id
4d1a0726-1e8f-482e-98bd-a1321c845ecd
date added to LUP
2017-04-10 10:28:12
date last changed
2017-11-19 04:39:10
@article{4d1a0726-1e8f-482e-98bd-a1321c845ecd,
  abstract     = {<p>In this work, the potential of combining capillary electrophoresis-time-of-flight-mass spectrome-try (CE-TOF-MS) and Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) for metabolomics of genetically modified organisms (GMOs) is demonstrated. Thus, six different varieties of maize, three of them transgenic (PR33P66 Bt, Tietar Bt and Aristis Bt) and their corresponding isogenic lines (PR33P66, Tietar and Aristis) grown under the same field conditions, were analyzed. Based on the ultrahigh resolution and remarkable mass accuracy provided by the 12-T FT-ICR-MS it was possible to directly analyze a good number of metabolites whose identity could be proposed based on their specific isotopic pattern. For identification of metabolite isomers, CE-TOF-MS was also used combining the information on nominal mass with electrophoretic mobility corroborating in that way the identity of several new biomarkers. Furthermore, PLE extractions were evaluated in order to establish selective extraction as an additional criterion to obtain useful information in maize metabolomics. Differences in the metabolite levels were found between the three transgenic maize varieties compared with their wild isogenic lines in some specific metabolic pathways. To our knowledge, this is the first time that an approach as the one presented in this work (pressurized liquid extraction + FT-ICR-MS + CE-TOF-MS) is shown for a metabolomic study.</p>},
  author       = {Leon, Emmanuel Carlos Dean and Rodriguez-Meizoso, Irene and Lucio, Marianna and Garcia-Cañas, Virginia and Ibañez, Elena and Schmitt-Kopplin, Philippe and Cifuentes, Alejandro},
  issn         = {0021-9673},
  keyword      = {Bt maize,Capillary electrophoresis-mass spectrometry (CE-MS),Corn,FT-MS,GMOs,Metabolites,Metabolomics,Transgenic foods},
  language     = {eng},
  month        = {10},
  number       = {43},
  pages        = {7314--7323},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Metabolomics of transgenic maize combining Fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extraction},
  url          = {http://dx.doi.org/10.1016/j.chroma.2009.04.092},
  volume       = {1216},
  year         = {2009},
}