Increased HPV detection by the use of a pre-heating step on vaginal self-samples analysed by Aptima HPV assay
(2019) In Journal of Virological Methods 270. p.18-20- Abstract
Background: We recently reported a sensitivity of 85.5% to detect high-grade squamous intraepithelial lesions (HSIL)/adenocarcinoma in situ (AIS)/cancer by the use of self-collected vaginal samples analysed by the Aptima mRNA HPV assay (AHPV). Objectives: To increase detection of HPV among self-samples. Study design: We used a pre-heating step at 90 °C for 1 h on our previously AHPV-negative self-samples (N = 20) among women with AHPV-positive cervical samples. We also analysed AHPV results before and after the heating among a series of self-samples from women who had not attended cervical screening for > 7 years (N = 173). Results: After heating, 55% (11/20) of the self-samples became AHPV-positive. By updating our original series... (More)
Background: We recently reported a sensitivity of 85.5% to detect high-grade squamous intraepithelial lesions (HSIL)/adenocarcinoma in situ (AIS)/cancer by the use of self-collected vaginal samples analysed by the Aptima mRNA HPV assay (AHPV). Objectives: To increase detection of HPV among self-samples. Study design: We used a pre-heating step at 90 °C for 1 h on our previously AHPV-negative self-samples (N = 20) among women with AHPV-positive cervical samples. We also analysed AHPV results before and after the heating among a series of self-samples from women who had not attended cervical screening for > 7 years (N = 173). Results: After heating, 55% (11/20) of the self-samples became AHPV-positive. By updating our original series 93.1% (121/130, 95% CI: 87.3–96.8) of the self-samples were AHPV-positive among women with AHPV-positive cervical samples, and among women with histologically confirmed cervical intraepithelial neoplasia or worse (CIN2+) now 95.3% (61/64, 95% CI: 86.9–99.0) of the self-samples were AHPV-positive. Among the 11 AHPV-positive self-samples we detected high-risk HPV types in 10 of the samples (HPV16 3 cases, HPV18 1, HPV31 1, HPV33 1, HPV 45 1, HPV51 2, HPV 56 and 58 1, HPV42 and 90 1 [low risk]) by multiplex PCR and Luminex assay. Among the self-samples from the non-attenders 16% (27/170) and 5.3% (8/152) were AHPV-positive after and before the heating step, respectively (P = 0.0022). Concerning validity of AHPV-results, 99% (170/172) were valid after the heating step compared to 88% (152/172) before the heating step (P < 0.0001). Conclusions: A pre-heating step on vaginal self-samples increased HPV detection by the AHPV assay.
(Less)
- author
- Borgfeldt, Christer LU and Forslund, Ola LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cancer, HPV mRNA, Screening, Self-sample, Vaginal
- in
- Journal of Virological Methods
- volume
- 270
- pages
- 3 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85064673913
- pmid:31002831
- ISSN
- 0166-0934
- DOI
- 10.1016/j.jviromet.2019.04.015
- language
- English
- LU publication?
- yes
- id
- 4d1e485f-cfc9-42ba-ac0f-6ad636d23109
- date added to LUP
- 2019-05-02 14:29:38
- date last changed
- 2024-07-09 11:02:03
@article{4d1e485f-cfc9-42ba-ac0f-6ad636d23109, abstract = {{<p>Background: We recently reported a sensitivity of 85.5% to detect high-grade squamous intraepithelial lesions (HSIL)/adenocarcinoma in situ (AIS)/cancer by the use of self-collected vaginal samples analysed by the Aptima mRNA HPV assay (AHPV). Objectives: To increase detection of HPV among self-samples. Study design: We used a pre-heating step at 90 °C for 1 h on our previously AHPV-negative self-samples (N = 20) among women with AHPV-positive cervical samples. We also analysed AHPV results before and after the heating among a series of self-samples from women who had not attended cervical screening for > 7 years (N = 173). Results: After heating, 55% (11/20) of the self-samples became AHPV-positive. By updating our original series 93.1% (121/130, 95% CI: 87.3–96.8) of the self-samples were AHPV-positive among women with AHPV-positive cervical samples, and among women with histologically confirmed cervical intraepithelial neoplasia or worse (CIN2+) now 95.3% (61/64, 95% CI: 86.9–99.0) of the self-samples were AHPV-positive. Among the 11 AHPV-positive self-samples we detected high-risk HPV types in 10 of the samples (HPV16 3 cases, HPV18 1, HPV31 1, HPV33 1, HPV 45 1, HPV51 2, HPV 56 and 58 1, HPV42 and 90 1 [low risk]) by multiplex PCR and Luminex assay. Among the self-samples from the non-attenders 16% (27/170) and 5.3% (8/152) were AHPV-positive after and before the heating step, respectively (P = 0.0022). Concerning validity of AHPV-results, 99% (170/172) were valid after the heating step compared to 88% (152/172) before the heating step (P < 0.0001). Conclusions: A pre-heating step on vaginal self-samples increased HPV detection by the AHPV assay.</p>}}, author = {{Borgfeldt, Christer and Forslund, Ola}}, issn = {{0166-0934}}, keywords = {{Cancer; HPV mRNA; Screening; Self-sample; Vaginal}}, language = {{eng}}, pages = {{18--20}}, publisher = {{Elsevier}}, series = {{Journal of Virological Methods}}, title = {{Increased HPV detection by the use of a pre-heating step on vaginal self-samples analysed by Aptima HPV assay}}, url = {{http://dx.doi.org/10.1016/j.jviromet.2019.04.015}}, doi = {{10.1016/j.jviromet.2019.04.015}}, volume = {{270}}, year = {{2019}}, }