A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme
(1973) In Eur J Biochem 40(1). p.187-193- Abstract
Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide].
The coenzymic activity of the two derivatives was tested with malate
dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The
efficiency relative to unsubstituted NAD+ was in the range of 50–100%.
NAD+-N6-[N-(6-aminohexyl)-acetamide] was attached to Sepharose 4B by the cyanogen bromide... (More)
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Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide].
The coenzymic activity of the two derivatives was tested with malate
dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The
efficiency relative to unsubstituted NAD+ was in the range of 50–100%.
NAD+-N6-[N-(6-aminohexyl)-acetamide] was attached to Sepharose 4B by the cyanogen bromide method. The immobilized NAD+
analogue thus obtained, exhibited cofactor activity when tested in a
recycling three-enzyme system (malate dehydrogenease-citrate
synthase-lactate dehydrogenase). The immobilized NAD+
analogue proved to be and effective ligand in affinity chromatography.
Thus a mixture of albumin, alcohol dehydrogenase and lactate
dehydrogenase was resolved with good recovery. The enzymes were eluted
with NAD+ and NADH, respectively.
Please use this url to cite or link to this publication: https://lup.lub.lu.se/record/4d367625-95cf-4e06-8c71-a3c3fdc743b1- author
- Lindberg, Margareta ; Larsson, Per-Olof LU and Mosbach, Klaus LU
- organization
- publishing date
- 1973
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Eur J Biochem
- volume
- 40
- issue
- 1
- pages
- 7 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0015730613
- ISSN
- 0014-2956
- DOI
- 10.1111/j.1432-1033.1973.tb03184.x
- language
- English
- LU publication?
- yes
- id
- 4d367625-95cf-4e06-8c71-a3c3fdc743b1
- date added to LUP
- 2024-06-22 21:01:09
- date last changed
- 2024-09-16 13:23:09
@article{4d367625-95cf-4e06-8c71-a3c3fdc743b1, abstract = {{<div class="article-section__content en main"><br> <p>Alkylation of NAD<sup>+</sup> with iodoacetic acid followed by alkaline rearrangement gave <i>N</i><sup>6</sup>-carboxymethyl-NAD<sup>+</sup>. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD<sup>+</sup>- <i>N</i><sup>6</sup>-[<i>N</i>-(6-aminohexyl)-acetamide].<br> The coenzymic activity of the two derivatives was tested with malate <br> dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The <br> efficiency relative to unsubstituted NAD<sup>+</sup> was in the range of 50–100%.</p><br> <p>NAD<sup>+</sup>-<i>N</i><sup>6</sup>-[<i>N</i>-(6-aminohexyl)-acetamide] was attached to Sepharose <b>4B</b> by the cyanogen bromide method. The immobilized NAD<sup>+</sup><br> analogue thus obtained, exhibited cofactor activity when tested in a <br> recycling three-enzyme system (malate dehydrogenease-citrate <br> synthase-lactate dehydrogenase). The immobilized NAD<sup>+</sup> <br> analogue proved to be and effective ligand in affinity chromatography. <br> Thus a mixture of albumin, alcohol dehydrogenase and lactate <br> dehydrogenase was resolved with good recovery. The enzymes were eluted <br> with NAD<sup>+</sup> and NADH, respectively.</p><br> </div>}}, author = {{Lindberg, Margareta and Larsson, Per-Olof and Mosbach, Klaus}}, issn = {{0014-2956}}, language = {{eng}}, number = {{1}}, pages = {{187--193}}, publisher = {{Wiley-Blackwell}}, series = {{Eur J Biochem}}, title = {{A New Immobilized NAD<sup>+</sup> Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme}}, url = {{http://dx.doi.org/10.1111/j.1432-1033.1973.tb03184.x}}, doi = {{10.1111/j.1432-1033.1973.tb03184.x}}, volume = {{40}}, year = {{1973}}, }