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A New Immobilized NAD+ Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme

Lindberg, Margareta ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1973) In Eur J Biochem 40(1). p.187-193
Abstract

Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide].
The coenzymic activity of the two derivatives was tested with malate
dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The
efficiency relative to unsubstituted NAD+ was in the range of 50–100%.


NAD+-N6-[N-(6-aminohexyl)-acetamide] was attached to Sepharose 4B by the cyanogen bromide... (More)


Alkylation of NAD+ with iodoacetic acid followed by alkaline rearrangement gave N6-carboxymethyl-NAD+. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD+- N6-[N-(6-aminohexyl)-acetamide].
The coenzymic activity of the two derivatives was tested with malate
dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The
efficiency relative to unsubstituted NAD+ was in the range of 50–100%.


NAD+-N6-[N-(6-aminohexyl)-acetamide] was attached to Sepharose 4B by the cyanogen bromide method. The immobilized NAD+
analogue thus obtained, exhibited cofactor activity when tested in a
recycling three-enzyme system (malate dehydrogenease-citrate
synthase-lactate dehydrogenase). The immobilized NAD+
analogue proved to be and effective ligand in affinity chromatography.
Thus a mixture of albumin, alcohol dehydrogenase and lactate
dehydrogenase was resolved with good recovery. The enzymes were eluted
with NAD+ and NADH, respectively.


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Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Eur J Biochem
volume
40
issue
1
pages
7 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0015730613
ISSN
0014-2956
DOI
10.1111/j.1432-1033.1973.tb03184.x
language
English
LU publication?
yes
id
4d367625-95cf-4e06-8c71-a3c3fdc743b1
date added to LUP
2024-06-22 21:01:09
date last changed
2024-09-16 13:23:09
@article{4d367625-95cf-4e06-8c71-a3c3fdc743b1,
  abstract     = {{<div class="article-section__content en main"><br>
            <p>Alkylation of NAD<sup>+</sup> with iodoacetic acid followed by alkaline rearrangement gave <i>N</i><sup>6</sup>-carboxymethyl-NAD<sup>+</sup>. Condensation of this analogue with 1,6-diaminohexane gave the analogue NAD<sup>+</sup>- <i>N</i><sup>6</sup>-[<i>N</i>-(6-aminohexyl)-acetamide].<br>
 The coenzymic activity of the two derivatives was tested with malate <br>
dehydrogenase, alcohol dehydrogenase and lactate dehydrogenase. The <br>
efficiency relative to unsubstituted NAD<sup>+</sup> was in the range of 50–100%.</p><br>
            <p>NAD<sup>+</sup>-<i>N</i><sup>6</sup>-[<i>N</i>-(6-aminohexyl)-acetamide] was attached to Sepharose <b>4B</b> by the cyanogen bromide method. The immobilized NAD<sup>+</sup><br>
 analogue thus obtained, exhibited cofactor activity when tested in a <br>
recycling three-enzyme system (malate dehydrogenease-citrate <br>
synthase-lactate dehydrogenase). The immobilized NAD<sup>+</sup> <br>
analogue proved to be and effective ligand in affinity chromatography. <br>
Thus a mixture of albumin, alcohol dehydrogenase and lactate <br>
dehydrogenase was resolved with good recovery. The enzymes were eluted <br>
with NAD<sup>+</sup> and NADH, respectively.</p><br>
            </div>}},
  author       = {{Lindberg, Margareta and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0014-2956}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{187--193}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Eur J Biochem}},
  title        = {{A New Immobilized NAD<sup>+</sup> Analogue, Its Application in Affinity Chromatography and as a Functioning Coenzyme}},
  url          = {{http://dx.doi.org/10.1111/j.1432-1033.1973.tb03184.x}},
  doi          = {{10.1111/j.1432-1033.1973.tb03184.x}},
  volume       = {{40}},
  year         = {{1973}},
}