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Global microRNA analysis of the NCI-60 cancer cell panel

Søkilde, Rolf LU ; Kaczkowski, Bogumil; Podolska, Agnieszka; Cirera, Susanna; Gorodkin, Jan; Møller, Søren and Litman, Thomas (2011) In Molecular Cancer Therapeutics 10(3). p.84-375
Abstract

MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 955 miRNAs in the NCI-60 cancer cell lines and identified tissue- and cell-type-specific miRNA patterns by unsupervised hierarchical clustering and statistical analysis. A comparison of our data to three previously published miRNA... (More)

MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 955 miRNAs in the NCI-60 cancer cell lines and identified tissue- and cell-type-specific miRNA patterns by unsupervised hierarchical clustering and statistical analysis. A comparison of our data to three previously published miRNA expression studies on the NCI-60 panel showed a remarkably high correlation between the different technical platforms. In addition, the current work contributes expression data for 369 miRNAs that have not previously been profiled. Finally, by matching drug sensitivity data for the NCI-60 cells to their miRNA expression profiles, we found numerous drug-miRNAs pairs, for which the miRNA expression and drug sensitivity profiles were highly correlated and thus represent potential candidates for further investigation of drug resistance and sensitivity mechanisms.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Antineoplastic Agents, Biomarkers, Tumor, Blotting, Northern, Cell Line, Tumor, Drug Discovery, Drug Resistance, Neoplasm, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, Neoplasms, RNA Interference, RNA Processing, Post-Transcriptional, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Journal Article, Research Support, Non-U.S. Gov't
in
Molecular Cancer Therapeutics
volume
10
issue
3
pages
10 pages
publisher
American Association for Cancer Research
external identifiers
  • scopus:79955759815
ISSN
1538-8514
DOI
10.1158/1535-7163.MCT-10-0605
language
English
LU publication?
no
id
4d82b29d-6d78-4570-948e-18a2ca716f21
date added to LUP
2017-09-01 14:31:36
date last changed
2017-09-08 14:56:40
@article{4d82b29d-6d78-4570-948e-18a2ca716f21,
  abstract     = {<p>MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 955 miRNAs in the NCI-60 cancer cell lines and identified tissue- and cell-type-specific miRNA patterns by unsupervised hierarchical clustering and statistical analysis. A comparison of our data to three previously published miRNA expression studies on the NCI-60 panel showed a remarkably high correlation between the different technical platforms. In addition, the current work contributes expression data for 369 miRNAs that have not previously been profiled. Finally, by matching drug sensitivity data for the NCI-60 cells to their miRNA expression profiles, we found numerous drug-miRNAs pairs, for which the miRNA expression and drug sensitivity profiles were highly correlated and thus represent potential candidates for further investigation of drug resistance and sensitivity mechanisms.</p>},
  author       = {Søkilde, Rolf and Kaczkowski, Bogumil and Podolska, Agnieszka and Cirera, Susanna and Gorodkin, Jan and Møller, Søren and Litman, Thomas},
  issn         = {1538-8514},
  keyword      = {Antineoplastic Agents,Biomarkers, Tumor,Blotting, Northern,Cell Line, Tumor,Drug Discovery,Drug Resistance, Neoplasm,Gene Expression Profiling,Gene Expression Regulation, Neoplastic,Humans,MicroRNAs,Neoplasms,RNA Interference,RNA Processing, Post-Transcriptional,RNA, Messenger,Reverse Transcriptase Polymerase Chain Reaction,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {84--375},
  publisher    = {American Association for Cancer Research},
  series       = {Molecular Cancer Therapeutics},
  title        = {Global microRNA analysis of the NCI-60 cancer cell panel},
  url          = {http://dx.doi.org/10.1158/1535-7163.MCT-10-0605},
  volume       = {10},
  year         = {2011},
}