Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.
(2002) In Journal of Microbiological Methods 48(2-3). p.267-270- Abstract
- The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106862
- author
- Bal, Karol LU ; Larsson, Lennart LU ; Mielniczuk, Eugenia and Mielniczuk, Zbigniew
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Muramic Acids : chemistry, Trimethylsilyl Compounds : chemistry, Spectrum Analysis Mass, Peptidoglycan : analysis, Bacteria : chemistry
- in
- Journal of Microbiological Methods
- volume
- 48
- issue
- 2-3
- pages
- 267 - 270
- publisher
- Elsevier
- external identifiers
-
- wos:000173483900016
- pmid:11777574
- scopus:0036147685
- ISSN
- 1872-8359
- DOI
- 10.1016/S0167-7012(01)00328-1
- language
- English
- LU publication?
- yes
- id
- 4dd67462-04e3-4d4d-8bdf-38ac8102c4a5 (old id 106862)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11777574&dopt=Abstract
- date added to LUP
- 2016-04-01 12:20:34
- date last changed
- 2022-01-27 02:18:50
@article{4dd67462-04e3-4d4d-8bdf-38ac8102c4a5, abstract = {{The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.}}, author = {{Bal, Karol and Larsson, Lennart and Mielniczuk, Eugenia and Mielniczuk, Zbigniew}}, issn = {{1872-8359}}, keywords = {{Muramic Acids : chemistry; Trimethylsilyl Compounds : chemistry; Spectrum Analysis Mass; Peptidoglycan : analysis; Bacteria : chemistry}}, language = {{eng}}, number = {{2-3}}, pages = {{267--270}}, publisher = {{Elsevier}}, series = {{Journal of Microbiological Methods}}, title = {{Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.}}, url = {{http://dx.doi.org/10.1016/S0167-7012(01)00328-1}}, doi = {{10.1016/S0167-7012(01)00328-1}}, volume = {{48}}, year = {{2002}}, }