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MRS glucose mapping and PET joining forces : re-evaluation of the lumped constant in the rat brain under isoflurane anaesthesia

Alf, Malte F; Duarte, João M N LU ; Lei, Hongxia; Krämer, Stefanie D; Mlynarik, Vladimir; Schibli, Roger and Gruetter, Rolf (2014) In Journal of Neurochemistry 129(4). p.82-672
Abstract

Although numerous positron emission tomography (PET) studies with (18) F-fluoro-deoxyglucose (FDG) have reported quantitative results on cerebral glucose kinetics and consumption, there is a large variation between the absolute values found in the literature. One of the underlying causes is the inconsistent use of the lumped constants (LCs), the derivation of which is often based on multiple assumptions that render absolute numbers imprecise and errors hard to quantify. We combined a kinetic FDG-PET study with magnetic resonance spectroscopic imaging (MRSI) of glucose dynamics in Sprague-Dawley rats to obtain a more comprehensive view of brain glucose kinetics and determine a reliable value for the LC under isoflurane anaesthesia. Maps... (More)

Although numerous positron emission tomography (PET) studies with (18) F-fluoro-deoxyglucose (FDG) have reported quantitative results on cerebral glucose kinetics and consumption, there is a large variation between the absolute values found in the literature. One of the underlying causes is the inconsistent use of the lumped constants (LCs), the derivation of which is often based on multiple assumptions that render absolute numbers imprecise and errors hard to quantify. We combined a kinetic FDG-PET study with magnetic resonance spectroscopic imaging (MRSI) of glucose dynamics in Sprague-Dawley rats to obtain a more comprehensive view of brain glucose kinetics and determine a reliable value for the LC under isoflurane anaesthesia. Maps of Tmax /CMRglc derived from MRSI data and Tmax determined from PET kinetic modelling allowed to obtain an LC-independent CMRglc . The LC was estimated to range from 0.33 ± 0.07 in retrosplenial cortex to 0.44 ± 0.05 in hippocampus, yielding CMRglc between 62 ± 14 and 54 ± 11 μmol/min/100 g, respectively. These newly determined LCs for four distinct areas in the rat brain under isoflurane anaesthesia provide means of comparing the growing amount of FDG-PET data available from translational studies.

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author
publishing date
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Contribution to journal
publication status
published
keywords
Algorithms, Anesthetics, Inhalation, Animals, Biological Transport, Brain, Brain Chemistry, Cerebral Cortex, Fluorine Radioisotopes, Fluorodeoxyglucose F18, Glucose, Hippocampus, Isoflurane, Magnetic Resonance Spectroscopy, Models, Biological, Multimodal Imaging, Positron-Emission Tomography, Radiopharmaceuticals, Rats, Rats, Sprague-Dawley, Thalamus, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurochemistry
volume
129
issue
4
pages
11 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:84899476604
ISSN
1471-4159
DOI
10.1111/jnc.12667
language
English
LU publication?
no
id
4dd779a6-5d63-4d13-b60c-ee015a45aa26
date added to LUP
2017-10-19 15:16:56
date last changed
2017-10-30 15:45:13
@article{4dd779a6-5d63-4d13-b60c-ee015a45aa26,
  abstract     = {<p>Although numerous positron emission tomography (PET) studies with (18) F-fluoro-deoxyglucose (FDG) have reported quantitative results on cerebral glucose kinetics and consumption, there is a large variation between the absolute values found in the literature. One of the underlying causes is the inconsistent use of the lumped constants (LCs), the derivation of which is often based on multiple assumptions that render absolute numbers imprecise and errors hard to quantify. We combined a kinetic FDG-PET study with magnetic resonance spectroscopic imaging (MRSI) of glucose dynamics in Sprague-Dawley rats to obtain a more comprehensive view of brain glucose kinetics and determine a reliable value for the LC under isoflurane anaesthesia. Maps of Tmax /CMRglc derived from MRSI data and Tmax determined from PET kinetic modelling allowed to obtain an LC-independent CMRglc . The LC was estimated to range from 0.33 ± 0.07 in retrosplenial cortex to 0.44 ± 0.05 in hippocampus, yielding CMRglc between 62 ± 14 and 54 ± 11 μmol/min/100 g, respectively. These newly determined LCs for four distinct areas in the rat brain under isoflurane anaesthesia provide means of comparing the growing amount of FDG-PET data available from translational studies.</p>},
  author       = {Alf, Malte F and Duarte, João M N and Lei, Hongxia and Krämer, Stefanie D and Mlynarik, Vladimir and Schibli, Roger and Gruetter, Rolf},
  issn         = {1471-4159},
  keyword      = {Algorithms,Anesthetics, Inhalation,Animals,Biological Transport,Brain,Brain Chemistry,Cerebral Cortex,Fluorine Radioisotopes,Fluorodeoxyglucose F18,Glucose,Hippocampus,Isoflurane,Magnetic Resonance Spectroscopy,Models, Biological,Multimodal Imaging,Positron-Emission Tomography,Radiopharmaceuticals,Rats,Rats, Sprague-Dawley,Thalamus,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {4},
  pages        = {82--672},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {MRS glucose mapping and PET joining forces : re-evaluation of the lumped constant in the rat brain under isoflurane anaesthesia},
  url          = {http://dx.doi.org/10.1111/jnc.12667},
  volume       = {129},
  year         = {2014},
}