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Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry

Gustafsson, E; Thorén, K; Larsson, T; Davidsson, P; Karlsson, K A and Nilsson, Carol LU (2001) In Rapid Communications in Mass Spectrometry 15(6). p.32-428
Abstract

Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a... (More)

Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Bacterial Proteins, Cell Fractionation, Electrophoresis, Gel, Two-Dimensional, Escherichia coli, Isoelectric Focusing, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Journal Article, Research Support, Non-U.S. Gov't
in
Rapid Communications in Mass Spectrometry
volume
15
issue
6
pages
32 - 428
publisher
John Wiley & Sons
external identifiers
  • scopus:0035063984
ISSN
0951-4198
DOI
10.1002/rcm.243
language
English
LU publication?
no
id
4e204a39-3a3e-4919-862c-71a3b024ea79
date added to LUP
2017-05-16 10:45:42
date last changed
2018-01-07 12:03:46
@article{4e204a39-3a3e-4919-862c-71a3b024ea79,
  abstract     = {<p>Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.</p>},
  author       = {Gustafsson, E and Thorén, K and Larsson, T and Davidsson, P and Karlsson, K A and Nilsson, Carol},
  issn         = {0951-4198},
  keyword      = {Bacterial Proteins,Cell Fractionation,Electrophoresis, Gel, Two-Dimensional,Escherichia coli,Isoelectric Focusing,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {6},
  pages        = {32--428},
  publisher    = {John Wiley & Sons},
  series       = {Rapid Communications in Mass Spectrometry},
  title        = {Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry},
  url          = {http://dx.doi.org/10.1002/rcm.243},
  volume       = {15},
  year         = {2001},
}