Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids.
Evilevitch, Alex LU
; Roos, Wouter H
; Ivanovska, Irena L
; Jeembaeva, Meerim
LU
; Jönsson, Bengt
LU
and Wuite, Gijs J L
(2011)
In Journal of Molecular Biology
405.
p.18-23
- Abstract
- Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage ϕ29 and herpes simplex virus type 1 particles.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1732547
- author
- Evilevitch, Alex
LU
; Roos, Wouter H
; Ivanovska, Irena L
; Jeembaeva, Meerim
LU
; Jönsson, Bengt
LU
and Wuite, Gijs J L
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Molecular Biology
- volume
- 405
- pages
- 18 - 23
- publisher
- Elsevier
- external identifiers
-
- wos:000286700800003
- pmid:21035458
- scopus:78650420324
- pmid:21035458
- ISSN
- 1089-8638
- DOI
- 10.1016/j.jmb.2010.10.039
- language
- English
- LU publication?
- yes
- id
- 4e6017dc-d9c8-4320-85fc-b435806e0268 (old id 1732547)
- date added to LUP
- 2016-04-01 14:13:07
- date last changed
- 2022-01-27 23:28:49
@article{4e6017dc-d9c8-4320-85fc-b435806e0268,
abstract = {{Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg(2+) and Sp(4+)). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage ϕ29 and herpes simplex virus type 1 particles.}},
author = {{Evilevitch, Alex and Roos, Wouter H and Ivanovska, Irena L and Jeembaeva, Meerim and Jönsson, Bengt and Wuite, Gijs J L}},
issn = {{1089-8638}},
language = {{eng}},
pages = {{18--23}},
publisher = {{Elsevier}},
series = {{Journal of Molecular Biology}},
title = {{Effects of Salts on Internal DNA Pressure and Mechanical Properties of Phage Capsids.}},
url = {{http://dx.doi.org/10.1016/j.jmb.2010.10.039}},
doi = {{10.1016/j.jmb.2010.10.039}},
volume = {{405}},
year = {{2011}},
}