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A general method for the immobilization of cells with preserved viability

Nilsson, Kjell LU ; Birnbaum, Staffan ; Flygare, Susanne ; Linse, Lotta ; Schröder, Ulf ; Jeppsson, Ulla LU ; Larsson, Per-Olof LU ; Mosbach, Klaus LU and Brodelius, Peter (1983) In European Journal of Applied Microbiology and Biotechnology 17. p.319-326
Abstract
Microbial, algal, plant and animal cells have been immobilized, with preserved viability, by entrapment in various matrices according to a new bead polymerization technique. The cell polymer/monomer mixture is kept suspended in a hydrophobic phase such as soy, paraffin, or silicon oil, tri-n-butylphosphate, or dibutyphtalate, which is compatible with the cells. The various monomers or polymers tested include agarose, agar, carrageenan, alginate, fibrin, and polyacrylamide. Furthermore, by adjustment of the stirring speed of the suspension, beads of desired diameter can easily be obtained. The entrapped cells are fully viable and biosynthetically active.
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell immobilization, microbial, algal, plant and animal cells, suspension polymerisation
in
European Journal of Applied Microbiology and Biotechnology
volume
17
pages
8 pages
publisher
Springer
external identifiers
  • scopus:0000498790
ISSN
0171-1741
DOI
10.1007/BF00499497
language
English
LU publication?
yes
id
4e8957fe-1db3-4388-837d-cc4e65367935
date added to LUP
2024-06-18 17:53:37
date last changed
2024-09-06 14:02:03
@article{4e8957fe-1db3-4388-837d-cc4e65367935,
  abstract     = {{Microbial, algal, plant and animal cells have been immobilized, with preserved viability, by entrapment in various matrices according to a new bead polymerization technique. The cell polymer/monomer mixture is kept suspended in a hydrophobic phase such as soy, paraffin, or silicon oil, tri-n-butylphosphate, or dibutyphtalate, which is compatible with the cells. The various monomers or polymers tested include agarose, agar, carrageenan, alginate, fibrin, and polyacrylamide. Furthermore, by adjustment of the stirring speed of the suspension, beads of desired diameter can easily be obtained. The entrapped cells are fully viable and biosynthetically active.}},
  author       = {{Nilsson, Kjell and Birnbaum, Staffan and Flygare, Susanne and Linse, Lotta and Schröder, Ulf and Jeppsson, Ulla and Larsson, Per-Olof and Mosbach, Klaus and Brodelius, Peter}},
  issn         = {{0171-1741}},
  keywords     = {{cell immobilization; microbial, algal, plant and animal cells; suspension polymerisation}},
  language     = {{eng}},
  pages        = {{319--326}},
  publisher    = {{Springer}},
  series       = {{European Journal of Applied Microbiology and Biotechnology}},
  title        = {{A general method for the immobilization of cells with preserved viability}},
  url          = {{http://dx.doi.org/10.1007/BF00499497}},
  doi          = {{10.1007/BF00499497}},
  volume       = {{17}},
  year         = {{1983}},
}