Advanced

Accuracy of determination of the glomerular filtration marker iohexol by European laboratories as monitored by external quality assessment

Nordin, Gunnar; Ekvall, Sara; Kristoffersson, Carolina; Jonsson, Ann Sofie; Bäck, Sten Erik LU ; Rollborn, Niclas and Larsson, Anders (2019) In Clinical Chemistry and Laboratory Medicine
Abstract

Glomerular filtration is the most important kidney function. The most accurate glomerular filtration rate (GFR) estimates are based on the clearance of exogenous filtration markers. Of these, iohexol is the only exogenous marker that is included in an external quality assessment (EQA) scheme. The aim of the present study was to evaluate the performance of the European laboratories participating in Equalis' EQA scheme for iohexol. Weighed amounts of iohexol (Omnipaque) were added to plasma samples and distributed to laboratories participating in the EQA scheme for iohexol. All laboratories performed the assays in a blinded fashion. The number of participating laboratories varied between 27 and 34 during the study period. Iohexol was... (More)

Glomerular filtration is the most important kidney function. The most accurate glomerular filtration rate (GFR) estimates are based on the clearance of exogenous filtration markers. Of these, iohexol is the only exogenous marker that is included in an external quality assessment (EQA) scheme. The aim of the present study was to evaluate the performance of the European laboratories participating in Equalis' EQA scheme for iohexol. Weighed amounts of iohexol (Omnipaque) were added to plasma samples and distributed to laboratories participating in the EQA scheme for iohexol. All laboratories performed the assays in a blinded fashion. The number of participating laboratories varied between 27 and 34 during the study period. Iohexol was determined by HPLC in 77% of the laboratories and by UPLC/MS/MS methods in 15% of the laboratories. The mean interlaboratory coefficient of variation was 4.7% for the HPLC methods and 6.4% for the UPLC/MS/MS methods. The mean bias between calculated and measured iohexol values was -1.3 mg/L (95% confidence interval ±0.3) during the first part of the study period and 0.1 mg/L (±0.3) during the later part. The low interlaboratory variation demonstrates that iohexol can be measured reliably by many laboratories and supports the use of iohexol as a GFR marker when there is a need for high quality GFR measurements.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
external quality assessment scheme, GFR, glomerular filtration rate, iohexol
in
Clinical Chemistry and Laboratory Medicine
publisher
De Gruyter
external identifiers
  • scopus:85065610984
ISSN
1434-6621
DOI
10.1515/cclm-2018-1175
language
English
LU publication?
no
id
4ea9098a-c263-4382-8c55-8961baa76556
date added to LUP
2019-06-04 12:32:42
date last changed
2019-06-19 04:14:41
@article{4ea9098a-c263-4382-8c55-8961baa76556,
  abstract     = {<p>Glomerular filtration is the most important kidney function. The most accurate glomerular filtration rate (GFR) estimates are based on the clearance of exogenous filtration markers. Of these, iohexol is the only exogenous marker that is included in an external quality assessment (EQA) scheme. The aim of the present study was to evaluate the performance of the European laboratories participating in Equalis' EQA scheme for iohexol. Weighed amounts of iohexol (Omnipaque) were added to plasma samples and distributed to laboratories participating in the EQA scheme for iohexol. All laboratories performed the assays in a blinded fashion. The number of participating laboratories varied between 27 and 34 during the study period. Iohexol was determined by HPLC in 77% of the laboratories and by UPLC/MS/MS methods in 15% of the laboratories. The mean interlaboratory coefficient of variation was 4.7% for the HPLC methods and 6.4% for the UPLC/MS/MS methods. The mean bias between calculated and measured iohexol values was -1.3 mg/L (95% confidence interval ±0.3) during the first part of the study period and 0.1 mg/L (±0.3) during the later part. The low interlaboratory variation demonstrates that iohexol can be measured reliably by many laboratories and supports the use of iohexol as a GFR marker when there is a need for high quality GFR measurements.</p>},
  author       = {Nordin, Gunnar and Ekvall, Sara and Kristoffersson, Carolina and Jonsson, Ann Sofie and Bäck, Sten Erik and Rollborn, Niclas and Larsson, Anders},
  issn         = {1434-6621},
  keyword      = {external quality assessment scheme,GFR,glomerular filtration rate,iohexol},
  language     = {eng},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Accuracy of determination of the glomerular filtration marker iohexol by European laboratories as monitored by external quality assessment},
  url          = {http://dx.doi.org/10.1515/cclm-2018-1175},
  year         = {2019},
}