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Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.

Erenpreiss, Juris LU ; Bungum, Mona LU ; Spano, M; Elzanaty, Saad LU ; Orbidans, J and Giwercman, Aleksander LU (2006) In Human Reproduction 21(2006 May 9). p.2061-2064
Abstract
BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion... (More)
BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI > 30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flow cytometry, sperm DNA integrity, SCSA, infertility, sperm chromatin
in
Human Reproduction
volume
21
issue
2006 May 9
pages
2061 - 2064
publisher
Oxford University Press
external identifiers
  • wos:000239901900021
  • pmid:16684843
  • scopus:33747877744
ISSN
0268-1161
DOI
10.1093/humrep/del134
language
English
LU publication?
yes
id
4ef20d23-610c-4396-b5d1-970e62dbafe7 (old id 156899)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16684843&dopt=Abstract
date added to LUP
2007-07-18 16:04:11
date last changed
2019-10-01 01:28:30
@article{4ef20d23-610c-4396-b5d1-970e62dbafe7,
  abstract     = {BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI &gt; 30% in the first test had DFI &lt; 30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI &gt; 30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated.},
  author       = {Erenpreiss, Juris and Bungum, Mona and Spano, M and Elzanaty, Saad and Orbidans, J and Giwercman, Aleksander},
  issn         = {0268-1161},
  keyword      = {flow cytometry,sperm DNA integrity,SCSA,infertility,sperm chromatin},
  language     = {eng},
  number       = {2006 May 9},
  pages        = {2061--2064},
  publisher    = {Oxford University Press},
  series       = {Human Reproduction},
  title        = {Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.},
  url          = {http://dx.doi.org/10.1093/humrep/del134},
  volume       = {21},
  year         = {2006},
}