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Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.

Schifferdecker, Anna LU ; Siurkas, Juozas ; Andersen, Mikael Rørdam ; Joerck-Ramberg, Dorte ; Ling, Zhihao ; Zhou, Nerve LU ; Blevins, James E ; Sibirny, Andriy A ; Piskur, Jure LU and Ishchuk, Olena LU (2016) In Applied Microbiology and Biotechnology
Abstract
Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic... (More)
Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering. (Less)
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type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
publisher
Springer
external identifiers
  • pmid:26743658
  • scopus:84960812995
  • wos:000372282000023
  • pmid:26743658
ISSN
1432-0614
DOI
10.1007/s00253-015-7266-x
language
English
LU publication?
yes
id
4f1d319c-b2ce-4a09-8c19-96b4bc1b6c08 (old id 8592729)
date added to LUP
2016-04-01 15:03:00
date last changed
2025-04-04 14:39:03
@article{4f1d319c-b2ce-4a09-8c19-96b4bc1b6c08,
  abstract     = {{Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering.}},
  author       = {{Schifferdecker, Anna and Siurkas, Juozas and Andersen, Mikael Rørdam and Joerck-Ramberg, Dorte and Ling, Zhihao and Zhou, Nerve and Blevins, James E and Sibirny, Andriy A and Piskur, Jure and Ishchuk, Olena}},
  issn         = {{1432-0614}},
  language     = {{eng}},
  month        = {{01}},
  publisher    = {{Springer}},
  series       = {{Applied Microbiology and Biotechnology}},
  title        = {{Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast.}},
  url          = {{http://dx.doi.org/10.1007/s00253-015-7266-x}},
  doi          = {{10.1007/s00253-015-7266-x}},
  year         = {{2016}},
}