Cell-cycle kinetics of proliferating mouse B lymphocytes in vitro
(1995) In Scandinavian Journal of Immunology 41(1). p.98-101- Abstract
Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of... (More)
Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.
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- author
- Källberg, E LU and Leanderson, T LU
- organization
- publishing date
- 1995-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Antibodies, Anti-Idiotypic/immunology, B-Lymphocytes/cytology, Cell Cycle/drug effects, Immunoglobulin kappa-Chains/immunology, Lipopolysaccharides/pharmacology, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Receptors, Antigen, B-Cell/physiology, Vinblastine/pharmacology
- in
- Scandinavian Journal of Immunology
- volume
- 41
- issue
- 1
- pages
- 4 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0028931256
- pmid:7824895
- ISSN
- 0300-9475
- DOI
- 10.1111/j.1365-3083.1995.tb03539.x
- language
- English
- LU publication?
- yes
- id
- 4f2088a0-a3de-45a0-913f-6e3542d1c188
- date added to LUP
- 2021-10-07 10:47:30
- date last changed
- 2024-01-05 17:48:00
@article{4f2088a0-a3de-45a0-913f-6e3542d1c188, abstract = {{<p>Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.</p>}}, author = {{Källberg, E and Leanderson, T}}, issn = {{0300-9475}}, keywords = {{Animals; Antibodies, Anti-Idiotypic/immunology; B-Lymphocytes/cytology; Cell Cycle/drug effects; Immunoglobulin kappa-Chains/immunology; Lipopolysaccharides/pharmacology; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Receptors, Antigen, B-Cell/physiology; Vinblastine/pharmacology}}, language = {{eng}}, number = {{1}}, pages = {{98--101}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{Cell-cycle kinetics of proliferating mouse B lymphocytes in vitro}}, url = {{http://dx.doi.org/10.1111/j.1365-3083.1995.tb03539.x}}, doi = {{10.1111/j.1365-3083.1995.tb03539.x}}, volume = {{41}}, year = {{1995}}, }