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Cell-cycle kinetics of proliferating mouse B lymphocytes in vitro

Källberg, E LU and Leanderson, T LU (1995) In Scandinavian Journal of Immunology 41(1). p.98-101
Abstract

Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of... (More)

Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.

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type
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publication status
published
subject
keywords
Animals, Antibodies, Anti-Idiotypic/immunology, B-Lymphocytes/cytology, Cell Cycle/drug effects, Immunoglobulin kappa-Chains/immunology, Lipopolysaccharides/pharmacology, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Receptors, Antigen, B-Cell/physiology, Vinblastine/pharmacology
in
Scandinavian Journal of Immunology
volume
41
issue
1
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0028931256
  • pmid:7824895
ISSN
0300-9475
DOI
10.1111/j.1365-3083.1995.tb03539.x
language
English
LU publication?
yes
id
4f2088a0-a3de-45a0-913f-6e3542d1c188
date added to LUP
2021-10-07 10:47:30
date last changed
2024-01-05 17:48:00
@article{4f2088a0-a3de-45a0-913f-6e3542d1c188,
  abstract     = {{<p>Resting mouse B lymphocytes were stimulated in vitro with lipopolysaccharide, Sepharose-coupled anti-kappa antibodies or a combination of the two. B lymphocytes stimulated with anti-kappa entered the cell-cycle with more rapid kinetics and at a higher frequency than did the corresponding cell population stimulated with lipopolysaccharide. Using cell cycle analysis after DNA staining combined with an M phase block, the cell-cycle kinetics of in vitro cultured B-lymphocytes was studied. The labelling index of lipopolysaccharide stimulated B lymphocytes was 60% while that for anti-kappa Sepharose stimulated cells was 85%. The generation time of the actively cycling population from both types of cultures was constant and was of the order of 18 h. Thus, the fraction of B lymphocytes induced to proliferate in vitro varies depending on the stimulus, while the growth kinetics of the actively proliferating populations are remarkably constant.</p>}},
  author       = {{Källberg, E and Leanderson, T}},
  issn         = {{0300-9475}},
  keywords     = {{Animals; Antibodies, Anti-Idiotypic/immunology; B-Lymphocytes/cytology; Cell Cycle/drug effects; Immunoglobulin kappa-Chains/immunology; Lipopolysaccharides/pharmacology; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Receptors, Antigen, B-Cell/physiology; Vinblastine/pharmacology}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{98--101}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Scandinavian Journal of Immunology}},
  title        = {{Cell-cycle kinetics of proliferating mouse B lymphocytes in vitro}},
  url          = {{http://dx.doi.org/10.1111/j.1365-3083.1995.tb03539.x}},
  doi          = {{10.1111/j.1365-3083.1995.tb03539.x}},
  volume       = {{41}},
  year         = {{1995}},
}