Steroid transformation in aqueous two-phase systems: side-chain degradation of cholesterol by Mycobacterium sp.
(1989) In Enzyme and Microbial Technology 11(11). p.752-759- Abstract
- We report on the use of aqueous two-phase systems for the side-chain degradation of cholesterol into androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD) by Mycobacterium sp. A problem encountered at the start of this investigation, extensive aggregation of the cells, was solved by careful choice of the polymer and detergent composition of the phase system. The systems showing the best properties were composed of polyethylene glycol, dextran and Brij 35 or polyvinylpyrrolidone, dextran and Brij 35 (or Tween 40). The bacterium maintained in the upper polyethylene glycol-rich (or polyvinylpyrrolidone-rich) phase was found to have high activity. The substrated partitioned to the upper phase or the interface (if present as... (More)
- We report on the use of aqueous two-phase systems for the side-chain degradation of cholesterol into androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD) by Mycobacterium sp. A problem encountered at the start of this investigation, extensive aggregation of the cells, was solved by careful choice of the polymer and detergent composition of the phase system. The systems showing the best properties were composed of polyethylene glycol, dextran and Brij 35 or polyvinylpyrrolidone, dextran and Brij 35 (or Tween 40). The bacterium maintained in the upper polyethylene glycol-rich (or polyvinylpyrrolidone-rich) phase was found to have high activity. The substrated partitioned to the upper phase or the interface (if present as crystals), while the products had partition coefficients around 2. (Less)
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- author
- Flygare, Susanne and Larsson, Per-Olof LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aqueous two-phase system, androsta-1,4-diene-3,17-dion, Mycobacterium sp.
- in
- Enzyme and Microbial Technology
- volume
- 11
- issue
- 11
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0024764152
- ISSN
- 0141-0229
- DOI
- 10.1016/0141-0229(89)90125-7
- language
- English
- LU publication?
- yes
- id
- 4f2af42a-3292-4008-afaf-3042725dce83
- date added to LUP
- 2024-06-18 13:30:48
- date last changed
- 2024-09-02 14:28:45
@article{4f2af42a-3292-4008-afaf-3042725dce83,
abstract = {{We report on the use of aqueous two-phase systems for the side-chain degradation of cholesterol into androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD) by Mycobacterium sp. A problem encountered at the start of this investigation, extensive aggregation of the cells, was solved by careful choice of the polymer and detergent composition of the phase system. The systems showing the best properties were composed of polyethylene glycol, dextran and Brij 35 or polyvinylpyrrolidone, dextran and Brij 35 (or Tween 40). The bacterium maintained in the upper polyethylene glycol-rich (or polyvinylpyrrolidone-rich) phase was found to have high activity. The substrated partitioned to the upper phase or the interface (if present as crystals), while the products had partition coefficients around 2.}},
author = {{Flygare, Susanne and Larsson, Per-Olof}},
issn = {{0141-0229}},
keywords = {{Aqueous two-phase system; androsta-1,4-diene-3,17-dion; Mycobacterium sp.}},
language = {{eng}},
number = {{11}},
pages = {{752--759}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Steroid transformation in aqueous two-phase systems: side-chain degradation of cholesterol by <i>Mycobacterium </i>sp.}},
url = {{http://dx.doi.org/10.1016/0141-0229(89)90125-7}},
doi = {{10.1016/0141-0229(89)90125-7}},
volume = {{11}},
year = {{1989}},
}