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Fluorescence signal enhancement in antibody microarrays using lightguiding nanowires

Verardo, Damiano LU ; Liljedahl, Leena LU ; Richter, Corinna LU ; Agnarsson, Björn ; Axelsson, Ulrika LU orcid ; Prinz, Christelle N. LU ; Höök, Fredrik LU ; Borrebaeck, Carl A.K. LU and Linke, Heiner LU orcid (2021) In Nanomaterials 11(1).
Abstract

Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding,... (More)

Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO2-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays.

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Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antibody microarray, Biomarker discovery, Nanowire sensors
in
Nanomaterials
volume
11
issue
1
article number
227
pages
11 pages
publisher
MDPI AG
external identifiers
  • pmid:33467141
  • scopus:85100193747
ISSN
2079-4991
DOI
10.3390/nano11010227
language
English
LU publication?
yes
id
4f679283-b321-4b26-8314-77b14c41983f
date added to LUP
2021-02-12 11:25:44
date last changed
2024-04-18 02:37:51
@article{4f679283-b321-4b26-8314-77b14c41983f,
  abstract     = {{<p>Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO<sub>2</sub>-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays.</p>}},
  author       = {{Verardo, Damiano and Liljedahl, Leena and Richter, Corinna and Agnarsson, Björn and Axelsson, Ulrika and Prinz, Christelle N. and Höök, Fredrik and Borrebaeck, Carl A.K. and Linke, Heiner}},
  issn         = {{2079-4991}},
  keywords     = {{Antibody microarray; Biomarker discovery; Nanowire sensors}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Nanomaterials}},
  title        = {{Fluorescence signal enhancement in antibody microarrays using lightguiding nanowires}},
  url          = {{http://dx.doi.org/10.3390/nano11010227}},
  doi          = {{10.3390/nano11010227}},
  volume       = {{11}},
  year         = {{2021}},
}