antibody microarray analysis of directly labelled proteomes
(2008) In Current Opinion in Biotechnology 19(1). p.55-61- Abstract
- In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic... (More)
- In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/809746
- author
- Wingren, Christer LU and Borrebaeck, Carl LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Current Opinion in Biotechnology
- volume
- 19
- issue
- 1
- pages
- 55 - 61
- publisher
- Elsevier
- external identifiers
-
- wos:000253767600010
- scopus:39149088290
- pmid:18187318
- ISSN
- 0958-1669
- DOI
- 10.1016/j.copbio.2007.11.010
- language
- English
- LU publication?
- yes
- id
- 4fb148f2-e9d0-4d8b-b1a6-de23c003f221 (old id 809746)
- date added to LUP
- 2016-04-01 13:15:09
- date last changed
- 2022-04-06 03:29:20
@article{4fb148f2-e9d0-4d8b-b1a6-de23c003f221,
abstract = {{In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented.}},
author = {{Wingren, Christer and Borrebaeck, Carl}},
issn = {{0958-1669}},
language = {{eng}},
number = {{1}},
pages = {{55--61}},
publisher = {{Elsevier}},
series = {{Current Opinion in Biotechnology}},
title = {{antibody microarray analysis of directly labelled proteomes}},
url = {{http://dx.doi.org/10.1016/j.copbio.2007.11.010}},
doi = {{10.1016/j.copbio.2007.11.010}},
volume = {{19}},
year = {{2008}},
}