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Genome-wide regulatory deterioration impedes adaptive responses to stress in inbred populations of Drosophila melanogaster : inbreeding under stress assessed by RNAseq

Schou, Mads F. LU ; Bechsgaard, Jesper ; Muñoz, Joaquin and Kristensen, Torsten N. (2018) In Evolution 72(8). p.1614-1628
Abstract
Inbreeding depression is often intensified under environmental stress (i.e., inbreeding–stress interaction). Although the fitness
consequences of this phenomenon are well-described, underlying mechanisms such as an increased expression of deleterious alleles
under stress, or a lower capacity for adaptive responses to stress with inbreeding, have rarely been investigated. We investigated a
fitness component (egg-to-adult viability) and gene-expression patterns using RNA-seq analyses in noninbred control lines and in
inbred lines of Drosophila melanogaster exposed to benign temperature or heat stress. We find little support for an increase in the
cumulative expression of deleterious alleles under stress. Instead, inbred... (More)
Inbreeding depression is often intensified under environmental stress (i.e., inbreeding–stress interaction). Although the fitness
consequences of this phenomenon are well-described, underlying mechanisms such as an increased expression of deleterious alleles
under stress, or a lower capacity for adaptive responses to stress with inbreeding, have rarely been investigated. We investigated a
fitness component (egg-to-adult viability) and gene-expression patterns using RNA-seq analyses in noninbred control lines and in
inbred lines of Drosophila melanogaster exposed to benign temperature or heat stress. We find little support for an increase in the
cumulative expression of deleterious alleles under stress. Instead, inbred individuals had a reduced ability to induce an adaptive
gene regulatory stress response compared to controls. The decrease in egg-to-adult viability due to stress was most pronounced in
the lines with the largest deviation in the adaptive stress response (R
2
= 0.48). Thus, we find strong evidence for a lower capacity
of inbred individuals to respond by gene regulation to stress and that this is the main driver of inbreeding-stress interactions. In
comparison, the altered gene expression due to inbreeding at benign temperature showed no correlation with fitness and was
pronounced in genomic regions experiencing the highest increase in homozygosity.
(Less)
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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inbreeding depression, inbreeding–stress interactions, small populations
in
Evolution
volume
72
issue
8
pages
1614 - 1628
publisher
Wiley-Blackwell
external identifiers
  • scopus:85053919753
  • pmid:29738620
ISSN
0014-3820
DOI
10.1111/evo.13497
language
English
LU publication?
no
id
4fb7b6cc-19c8-41fb-899d-dc4e7981822a
date added to LUP
2018-09-11 13:59:47
date last changed
2022-03-17 17:16:23
@article{4fb7b6cc-19c8-41fb-899d-dc4e7981822a,
  abstract     = {{Inbreeding depression is often intensified under environmental stress (i.e., inbreeding–stress interaction). Although the fitness<br/>consequences of this phenomenon are well-described, underlying mechanisms such as an increased expression of deleterious alleles<br/>under stress, or a lower capacity for adaptive responses to stress with inbreeding, have rarely been investigated. We investigated a<br/>fitness component (egg-to-adult viability) and gene-expression patterns using RNA-seq analyses in noninbred control lines and in<br/>inbred lines of Drosophila melanogaster exposed to benign temperature or heat stress. We find little support for an increase in the<br/>cumulative expression of deleterious alleles under stress. Instead, inbred individuals had a reduced ability to induce an adaptive<br/>gene regulatory stress response compared to controls. The decrease in egg-to-adult viability due to stress was most pronounced in<br/>the lines with the largest deviation in the adaptive stress response (R<br/>2<br/>= 0.48). Thus, we find strong evidence for a lower capacity<br/>of inbred individuals to respond by gene regulation to stress and that this is the main driver of inbreeding-stress interactions. In<br/>comparison, the altered gene expression due to inbreeding at benign temperature showed no correlation with fitness and was<br/>pronounced in genomic regions experiencing the highest increase in homozygosity.<br/>}},
  author       = {{Schou, Mads F. and Bechsgaard, Jesper and Muñoz, Joaquin and Kristensen, Torsten N.}},
  issn         = {{0014-3820}},
  keywords     = {{Inbreeding depression; inbreeding–stress interactions; small populations}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{8}},
  pages        = {{1614--1628}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Evolution}},
  title        = {{Genome-wide regulatory deterioration impedes adaptive responses to stress in inbred populations of Drosophila melanogaster : inbreeding under stress assessed by RNAseq}},
  url          = {{http://dx.doi.org/10.1111/evo.13497}},
  doi          = {{10.1111/evo.13497}},
  volume       = {{72}},
  year         = {{2018}},
}