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Rapid intra-operative diagnosis of kidney cancer by attenuated total reflection infrared spectroscopy of tissue smears

Pucetaite, Milda LU ; Velicka, Martynas; Urboniene, Vidita; Ceponkus, Justinas; Bandzeviciute, Rimante; Jankevicius, Feliksas; Zelvys, Arunas; Sablinskas, Valdas and Steiner, Gerald (2018) In Journal of Biophotonics 11(5).
Abstract

Herein, a technique to analyze air-dried kidney tissue impression smears by means of attenuated total reflection infrared (ATR-IR) spectroscopy is presented. Spectral tumor markers-absorption bands of glycogen-are identified in the ATR-IR spectra of the kidney tissue smear samples. Thin kidney tissue cryo-sections currently used for IR spectroscopic analysis lack such spectral markers as the sample preparation causes irreversible molecular changes in the tissue. In particular, freeze-thaw cycle results in degradation of the glycogen and reduction or complete dissolution of its content. Supervised spectral classification was applied to the recorded spectra of the smears and the test spectra were classified with a high accuracy of 92% for... (More)

Herein, a technique to analyze air-dried kidney tissue impression smears by means of attenuated total reflection infrared (ATR-IR) spectroscopy is presented. Spectral tumor markers-absorption bands of glycogen-are identified in the ATR-IR spectra of the kidney tissue smear samples. Thin kidney tissue cryo-sections currently used for IR spectroscopic analysis lack such spectral markers as the sample preparation causes irreversible molecular changes in the tissue. In particular, freeze-thaw cycle results in degradation of the glycogen and reduction or complete dissolution of its content. Supervised spectral classification was applied to the recorded spectra of the smears and the test spectra were classified with a high accuracy of 92% for normal tissue and 94% for tumor tissue, respectively. For further development, we propose that combination of the method with optical fiber ATR probes could potentially be used for rapid real-time intra-operative tissue analysis without interfering with either the established protocols of pathological examination or the ordinary workflow of operating surgeon. Such approach could ensure easier transition of the method to clinical applications where it may complement the results of gold standard histopathology examination and aid in more precise resection of kidney tumors.

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author
publishing date
type
Contribution to journal
publication status
published
in
Journal of Biophotonics
volume
11
issue
5
pages
9 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:85042450728
ISSN
1864-063X
DOI
10.1002/jbio.201700260
language
English
LU publication?
no
id
4fc2780d-fe09-4714-a5db-44b935601066
date added to LUP
2019-03-18 11:47:28
date last changed
2019-05-17 19:06:35
@article{4fc2780d-fe09-4714-a5db-44b935601066,
  abstract     = {<p>Herein, a technique to analyze air-dried kidney tissue impression smears by means of attenuated total reflection infrared (ATR-IR) spectroscopy is presented. Spectral tumor markers-absorption bands of glycogen-are identified in the ATR-IR spectra of the kidney tissue smear samples. Thin kidney tissue cryo-sections currently used for IR spectroscopic analysis lack such spectral markers as the sample preparation causes irreversible molecular changes in the tissue. In particular, freeze-thaw cycle results in degradation of the glycogen and reduction or complete dissolution of its content. Supervised spectral classification was applied to the recorded spectra of the smears and the test spectra were classified with a high accuracy of 92% for normal tissue and 94% for tumor tissue, respectively. For further development, we propose that combination of the method with optical fiber ATR probes could potentially be used for rapid real-time intra-operative tissue analysis without interfering with either the established protocols of pathological examination or the ordinary workflow of operating surgeon. Such approach could ensure easier transition of the method to clinical applications where it may complement the results of gold standard histopathology examination and aid in more precise resection of kidney tumors.</p>},
  articleno    = {e201700260},
  author       = {Pucetaite, Milda and Velicka, Martynas and Urboniene, Vidita and Ceponkus, Justinas and Bandzeviciute, Rimante and Jankevicius, Feliksas and Zelvys, Arunas and Sablinskas, Valdas and Steiner, Gerald},
  issn         = {1864-063X},
  language     = {eng},
  number       = {5},
  pages        = {9},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Biophotonics},
  title        = {Rapid intra-operative diagnosis of kidney cancer by attenuated total reflection infrared spectroscopy of tissue smears},
  url          = {http://dx.doi.org/10.1002/jbio.201700260},
  volume       = {11},
  year         = {2018},
}