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Plant nonsense-mediated mRNA decay is controlled by different autoregulatory circuits and can be induced by an EJC-like complex

Nyikó, Tünde ; Kerényi, Farkas ; Szabadkai, Levente ; Benkovics, Anna H ; Major, Péter ; Sonkoly, Boglárka ; Mérai, Zsuzsanna ; Barta, Endre ; Niemiec, Emilia LU orcid and Kufel, Joanna , et al. (2013) In Nucleic Acids Research 41(13). p.28-6715
Abstract

Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that recognizes and degrades transcripts containing NMD cis elements in their 3'untranslated region (UTR). In yeasts, unusually long 3'UTRs act as NMD cis elements, whereas in vertebrates, NMD is induced by introns located >50 nt downstream from the stop codon. In vertebrates, splicing leads to deposition of exon junction complex (EJC) onto the mRNA, and then 3'UTR-bound EJCs trigger NMD. It is proposed that this intron-based NMD is vertebrate specific, and it evolved to eliminate the misproducts of alternative splicing. Here, we provide evidence that similar EJC-mediated intron-based NMD functions in plants, suggesting that this type of NMD is evolutionary... (More)

Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that recognizes and degrades transcripts containing NMD cis elements in their 3'untranslated region (UTR). In yeasts, unusually long 3'UTRs act as NMD cis elements, whereas in vertebrates, NMD is induced by introns located >50 nt downstream from the stop codon. In vertebrates, splicing leads to deposition of exon junction complex (EJC) onto the mRNA, and then 3'UTR-bound EJCs trigger NMD. It is proposed that this intron-based NMD is vertebrate specific, and it evolved to eliminate the misproducts of alternative splicing. Here, we provide evidence that similar EJC-mediated intron-based NMD functions in plants, suggesting that this type of NMD is evolutionary conserved. We demonstrate that in plants, like in vertebrates, introns located >50 nt from the stop induces NMD. We show that orthologs of all core EJC components are essential for intron-based plant NMD and that plant Partner of Y14 and mago (PYM) also acts as EJC disassembly factor. Moreover, we found that complex autoregulatory circuits control the activity of plant NMD. We demonstrate that expression of suppressor with morphogenic effect on genitalia (SMG)7, which is essential for long 3'UTR- and intron-based NMD, is regulated by both types of NMD, whereas expression of Barentsz EJC component is downregulated by intron-based NMD.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
3' Untranslated Regions, Arabidopsis Proteins/genetics, Carrier Proteins/genetics, Codon, Terminator, Gene Expression Regulation, Plant, Homeostasis, Introns, Nonsense Mediated mRNA Decay, Plant Proteins/physiology, RNA-Binding Proteins/metabolism
in
Nucleic Acids Research
volume
41
issue
13
pages
14 pages
publisher
Oxford University Press
external identifiers
  • scopus:84880534583
  • pmid:23666629
ISSN
1362-4962
DOI
10.1093/nar/gkt366
language
English
LU publication?
no
additional info
Copyright © 2013, Oxford University Press
id
5002372a-5d16-41e2-8c61-123cf535c7e7
date added to LUP
2020-11-17 17:34:40
date last changed
2024-03-05 14:53:54
@article{5002372a-5d16-41e2-8c61-123cf535c7e7,
  abstract     = {{<p>Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that recognizes and degrades transcripts containing NMD cis elements in their 3'untranslated region (UTR). In yeasts, unusually long 3'UTRs act as NMD cis elements, whereas in vertebrates, NMD is induced by introns located &gt;50 nt downstream from the stop codon. In vertebrates, splicing leads to deposition of exon junction complex (EJC) onto the mRNA, and then 3'UTR-bound EJCs trigger NMD. It is proposed that this intron-based NMD is vertebrate specific, and it evolved to eliminate the misproducts of alternative splicing. Here, we provide evidence that similar EJC-mediated intron-based NMD functions in plants, suggesting that this type of NMD is evolutionary conserved. We demonstrate that in plants, like in vertebrates, introns located &gt;50 nt from the stop induces NMD. We show that orthologs of all core EJC components are essential for intron-based plant NMD and that plant Partner of Y14 and mago (PYM) also acts as EJC disassembly factor. Moreover, we found that complex autoregulatory circuits control the activity of plant NMD. We demonstrate that expression of suppressor with morphogenic effect on genitalia (SMG)7, which is essential for long 3'UTR- and intron-based NMD, is regulated by both types of NMD, whereas expression of Barentsz EJC component is downregulated by intron-based NMD. </p>}},
  author       = {{Nyikó, Tünde and Kerényi, Farkas and Szabadkai, Levente and Benkovics, Anna H and Major, Péter and Sonkoly, Boglárka and Mérai, Zsuzsanna and Barta, Endre and Niemiec, Emilia and Kufel, Joanna and Silhavy, Dániel}},
  issn         = {{1362-4962}},
  keywords     = {{3' Untranslated Regions; Arabidopsis Proteins/genetics; Carrier Proteins/genetics; Codon, Terminator; Gene Expression Regulation, Plant; Homeostasis; Introns; Nonsense Mediated mRNA Decay; Plant Proteins/physiology; RNA-Binding Proteins/metabolism}},
  language     = {{eng}},
  number       = {{13}},
  pages        = {{28--6715}},
  publisher    = {{Oxford University Press}},
  series       = {{Nucleic Acids Research}},
  title        = {{Plant nonsense-mediated mRNA decay is controlled by different autoregulatory circuits and can be induced by an EJC-like complex}},
  url          = {{http://dx.doi.org/10.1093/nar/gkt366}},
  doi          = {{10.1093/nar/gkt366}},
  volume       = {{41}},
  year         = {{2013}},
}