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Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae.

Hosseini, S; Resjö, S; Liu, Yongfeng; Durling, M; Heyman, F; Levander, Fredrik LU ; Liu, Yanhong; Elfstrand, M; Funck Jensen, D and Andreasson, E, et al. (2015) In Journal of Proteomics 117. p.24-40
Abstract
The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using tandem mass spectrometry in a global proteome study of hyphae and germinating cysts in P. pisi and P. sojae. In total 2775 proteins from P. pisi and 2891 proteins from P. sojae were identified. Fifty-eight orthologous proteins were more abundant in germinated cysts of both pathogens and thus identified as candidate proteins for the infective stage. Several of these proteins were associated with... (More)
The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using tandem mass spectrometry in a global proteome study of hyphae and germinating cysts in P. pisi and P. sojae. In total 2775 proteins from P. pisi and 2891 proteins from P. sojae were identified. Fifty-eight orthologous proteins were more abundant in germinated cysts of both pathogens and thus identified as candidate proteins for the infective stage. Several of these proteins were associated with lipid transport and metabolism, and energy production. Twenty-three orthologous proteins were more abundant in hyphae of both pathogens and thus identified as candidate proteins for vegetative growth. Proteins uniquely present in germinating cysts of either P. pisi or P. sojae were considered as candidates for species-specific pathogenicity factors that may be involved in host specificity. Among these proteins were serine proteases, membrane transporters and a berberine-like protein. These results significantly expand the knowledge of the expressed proteome in P. pisi and P. sojae. (Less)
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Journal of Proteomics
volume
117
pages
24 - 40
publisher
Elsevier
external identifiers
  • pmid:25613045
  • wos:000350937700003
  • scopus:84923039347
ISSN
1874-3919
DOI
10.1016/j.jprot.2015.01.006
language
English
LU publication?
yes
id
20045f2f-9c7a-4875-ab53-d5e59c885aaa (old id 5039992)
date added to LUP
2015-02-16 14:00:24
date last changed
2017-03-19 03:14:48
@article{20045f2f-9c7a-4875-ab53-d5e59c885aaa,
  abstract     = {The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using tandem mass spectrometry in a global proteome study of hyphae and germinating cysts in P. pisi and P. sojae. In total 2775 proteins from P. pisi and 2891 proteins from P. sojae were identified. Fifty-eight orthologous proteins were more abundant in germinated cysts of both pathogens and thus identified as candidate proteins for the infective stage. Several of these proteins were associated with lipid transport and metabolism, and energy production. Twenty-three orthologous proteins were more abundant in hyphae of both pathogens and thus identified as candidate proteins for vegetative growth. Proteins uniquely present in germinating cysts of either P. pisi or P. sojae were considered as candidates for species-specific pathogenicity factors that may be involved in host specificity. Among these proteins were serine proteases, membrane transporters and a berberine-like protein. These results significantly expand the knowledge of the expressed proteome in P. pisi and P. sojae.},
  author       = {Hosseini, S and Resjö, S and Liu, Yongfeng and Durling, M and Heyman, F and Levander, Fredrik and Liu, Yanhong and Elfstrand, M and Funck Jensen, D and Andreasson, E and Karlsson, M},
  issn         = {1874-3919},
  language     = {eng},
  pages        = {24--40},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics},
  title        = {Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae.},
  url          = {http://dx.doi.org/10.1016/j.jprot.2015.01.006},
  volume       = {117},
  year         = {2015},
}