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Protein Unfolding allow use of Commercial Antibodies in an Apolipoprotein M sandwich ELISA.

Bosteen, Markus Hoeybye; Dahlbäck, Björn LU ; Nielsen, Lars Bo and Christoffersen, Christina (2015) In Journal of Lipid Research 56(3). p.754-759
Abstract
Apolipoprotein M (apoM) is a member of the lipocalin super family and circulates in plasma attached to high density lipoprotein particles. ApoM plays a role in cholesterol metabolism and has recently been identified as transporter for the signaling lipid Sphingosine-1-Phosphate (S1P) in plasma. S1P is implicated in several inflammatory diseases such as Multiple Sclerosis and Rheumatoid Arthritis. The ability to accurately measure apoM is crucial for investigating its biological functions and possible clinical implications. However, reliable commercial methods have been lacking so far. Therefore we have developed an assay that specifically recognizes human apoM in plasma using commercially available reagents. Commercial apoM antibodies were... (More)
Apolipoprotein M (apoM) is a member of the lipocalin super family and circulates in plasma attached to high density lipoprotein particles. ApoM plays a role in cholesterol metabolism and has recently been identified as transporter for the signaling lipid Sphingosine-1-Phosphate (S1P) in plasma. S1P is implicated in several inflammatory diseases such as Multiple Sclerosis and Rheumatoid Arthritis. The ability to accurately measure apoM is crucial for investigating its biological functions and possible clinical implications. However, reliable commercial methods have been lacking so far. Therefore we have developed an assay that specifically recognizes human apoM in plasma using commercially available reagents. Commercial apoM antibodies were screened for compatibility in a sandwich ELISA-based assay. One optimal pair of antibodies was chosen and sample preparation, buffers and incubation times were optimized to generate a simple and easily reproducible method. Validation and comparison to a previously described ELISA for apoM confirmed that the assay displays a high degree of sensitivity, specificity and precision. Our results show that commercially available antibodies can be used to accurately measure human plasma apoM. This method can be implemented in every laboratory and will help promote high quality research. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Lipid Research
volume
56
issue
3
pages
754 - 759
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • pmid:25561460
  • wos:000350345900027
  • scopus:84925307845
ISSN
1539-7262
DOI
10.1194/jlr.D055947
language
English
LU publication?
yes
id
33a12755-cee9-40f9-9865-b5a7b898fc69 (old id 5041143)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25561460?dopt=Abstract
date added to LUP
2015-02-02 17:47:28
date last changed
2017-11-19 03:04:42
@article{33a12755-cee9-40f9-9865-b5a7b898fc69,
  abstract     = {Apolipoprotein M (apoM) is a member of the lipocalin super family and circulates in plasma attached to high density lipoprotein particles. ApoM plays a role in cholesterol metabolism and has recently been identified as transporter for the signaling lipid Sphingosine-1-Phosphate (S1P) in plasma. S1P is implicated in several inflammatory diseases such as Multiple Sclerosis and Rheumatoid Arthritis. The ability to accurately measure apoM is crucial for investigating its biological functions and possible clinical implications. However, reliable commercial methods have been lacking so far. Therefore we have developed an assay that specifically recognizes human apoM in plasma using commercially available reagents. Commercial apoM antibodies were screened for compatibility in a sandwich ELISA-based assay. One optimal pair of antibodies was chosen and sample preparation, buffers and incubation times were optimized to generate a simple and easily reproducible method. Validation and comparison to a previously described ELISA for apoM confirmed that the assay displays a high degree of sensitivity, specificity and precision. Our results show that commercially available antibodies can be used to accurately measure human plasma apoM. This method can be implemented in every laboratory and will help promote high quality research.},
  author       = {Bosteen, Markus Hoeybye and Dahlbäck, Björn and Nielsen, Lars Bo and Christoffersen, Christina},
  issn         = {1539-7262},
  language     = {eng},
  number       = {3},
  pages        = {754--759},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Journal of Lipid Research},
  title        = {Protein Unfolding allow use of Commercial Antibodies in an Apolipoprotein M sandwich ELISA.},
  url          = {http://dx.doi.org/10.1194/jlr.D055947},
  volume       = {56},
  year         = {2015},
}