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Direct and mediated electrochemistry of peroxidase and its electrocatalysis on a variety of screen-printed carbon electrodes: amperometric hydrogen peroxide and phenols biosensor

Chekin, Fereshteh; Gorton, Lo LU and Tapsobea, Issa (2015) In Analytical and Bioanalytical Chemistry 407(2). p.439-446
Abstract
This study compares the behaviour of direct and mediated electrochemistry of horseradish peroxidase (HRP) immobilised on screen-printed carbon electrodes (SPCEs), screen-printed carbon electrodes modified with carboxyl-functionalised multi-wall carbon nanotubes (MWCNT-SPCEs) and screen-printed carbon electrodes modified with carboxyl-functionalised single-wall carbon nanotubes (SWCNT-SPCEs). The techniques of cyclic voltammetry and amperometry in the flow mode were used to characterise the properties of the HRP immobilised on screen-printed electrodes. From measurements of the mediated and mediatorless currents of hydrogen peroxide reduction at the HRP-modified electrodes, it was concluded that the fraction of enzyme molecules in direct... (More)
This study compares the behaviour of direct and mediated electrochemistry of horseradish peroxidase (HRP) immobilised on screen-printed carbon electrodes (SPCEs), screen-printed carbon electrodes modified with carboxyl-functionalised multi-wall carbon nanotubes (MWCNT-SPCEs) and screen-printed carbon electrodes modified with carboxyl-functionalised single-wall carbon nanotubes (SWCNT-SPCEs). The techniques of cyclic voltammetry and amperometry in the flow mode were used to characterise the properties of the HRP immobilised on screen-printed electrodes. From measurements of the mediated and mediatorless currents of hydrogen peroxide reduction at the HRP-modified electrodes, it was concluded that the fraction of enzyme molecules in direct electron transfer (DET) contact with the electrode varies substantially for the different electrodes. It was observed that the screen-printed carbon electrodes modified with carbon nanotubes (MWCNT-SPCEs and SWCNT-SPCEs) demonstrated a substantially higher percentage (a parts per thousand 100 %) of HRP molecules in DET contact than the screen-printed carbon electrodes (a parts per thousand 60 %). The HRP-modified electrodes were used for determination of hydrogen peroxide in mediatorless mode. The SWCNT-SPCE gave the lowest detection limit (0.40 +/- 0.09 mu M) followed by MWCNT-SPCE (0.48 +/- 0.07 mu M) and SPCE (0.98 +/- 0.2 mu M). These modified electrodes were additionally developed for amperometric determination of phenolic compounds. It was found that the SWCNT-SPCE gave a detection limit for catechol of 110.2 +/- 3.6 nM, dopamine of 640.2 +/- 9.2 nM, octopamine of 3341 +/- 15 nM, pyrogallol of 50.10 +/- 2.9 nM and 3,4-dihydroxy-l-phenylalanine of 980.7 +/- 8.7 nM using 50 mu M H2O2 in the flow carrier. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Screen-printed carbon electrodes, Peroxidase, Hydrogen peroxide, Phenolic compounds
in
Analytical and Bioanalytical Chemistry
volume
407
issue
2
pages
439 - 446
publisher
Springer
external identifiers
  • wos:000348207200008
  • scopus:84934334774
ISSN
1618-2642
DOI
10.1007/s00216-014-8282-x
language
English
LU publication?
yes
id
30d0482f-c3ac-4183-8bb2-8c61964123e7 (old id 5063092)
date added to LUP
2015-02-24 11:53:09
date last changed
2017-11-19 03:14:55
@article{30d0482f-c3ac-4183-8bb2-8c61964123e7,
  abstract     = {This study compares the behaviour of direct and mediated electrochemistry of horseradish peroxidase (HRP) immobilised on screen-printed carbon electrodes (SPCEs), screen-printed carbon electrodes modified with carboxyl-functionalised multi-wall carbon nanotubes (MWCNT-SPCEs) and screen-printed carbon electrodes modified with carboxyl-functionalised single-wall carbon nanotubes (SWCNT-SPCEs). The techniques of cyclic voltammetry and amperometry in the flow mode were used to characterise the properties of the HRP immobilised on screen-printed electrodes. From measurements of the mediated and mediatorless currents of hydrogen peroxide reduction at the HRP-modified electrodes, it was concluded that the fraction of enzyme molecules in direct electron transfer (DET) contact with the electrode varies substantially for the different electrodes. It was observed that the screen-printed carbon electrodes modified with carbon nanotubes (MWCNT-SPCEs and SWCNT-SPCEs) demonstrated a substantially higher percentage (a parts per thousand 100 %) of HRP molecules in DET contact than the screen-printed carbon electrodes (a parts per thousand 60 %). The HRP-modified electrodes were used for determination of hydrogen peroxide in mediatorless mode. The SWCNT-SPCE gave the lowest detection limit (0.40 +/- 0.09 mu M) followed by MWCNT-SPCE (0.48 +/- 0.07 mu M) and SPCE (0.98 +/- 0.2 mu M). These modified electrodes were additionally developed for amperometric determination of phenolic compounds. It was found that the SWCNT-SPCE gave a detection limit for catechol of 110.2 +/- 3.6 nM, dopamine of 640.2 +/- 9.2 nM, octopamine of 3341 +/- 15 nM, pyrogallol of 50.10 +/- 2.9 nM and 3,4-dihydroxy-l-phenylalanine of 980.7 +/- 8.7 nM using 50 mu M H2O2 in the flow carrier.},
  author       = {Chekin, Fereshteh and Gorton, Lo and Tapsobea, Issa},
  issn         = {1618-2642},
  keyword      = {Screen-printed carbon electrodes,Peroxidase,Hydrogen peroxide,Phenolic compounds},
  language     = {eng},
  number       = {2},
  pages        = {439--446},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Direct and mediated electrochemistry of peroxidase and its electrocatalysis on a variety of screen-printed carbon electrodes: amperometric hydrogen peroxide and phenols biosensor},
  url          = {http://dx.doi.org/10.1007/s00216-014-8282-x},
  volume       = {407},
  year         = {2015},
}