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Electroconvulsive treatment evokes release of preprotachykinin-A mRNA into the cerebrospinal fluid and ocular aqueous humor of rabbits

Wang, Z Y ; Monstein, H J ; Waldeck, K ; Grundemar, L LU and Håkanson, R LU (1997) In Neuroscience Letters 226(3). p.151-154
Abstract

Following electroconvulsive treatment (ECT) of rabbits, preprotachykinin-A (PPT-A) mRNA was detected by Southern blot analysis of polymerase chain reaction (PCR)-amplified products in the cerebrospinal fluid (CSF) and aqueous humor of the eye. In contrast, no PPT-A mRNA could be detected in samples from untreated animals. In addition, several neuropeptides (substance P, neuropeptide Y, cholecystokinin, calcitonin gene-related peptide and pituitary adenylate cyclase activating peptide) were released into the CSF (and aqueous humor) following ECT. The results suggest that PPT-A mRNA was released together with neuropeptides into the CSF and aqueous humor in response to ECT. Indeed, previous studies have suggested that neurons can release... (More)

Following electroconvulsive treatment (ECT) of rabbits, preprotachykinin-A (PPT-A) mRNA was detected by Southern blot analysis of polymerase chain reaction (PCR)-amplified products in the cerebrospinal fluid (CSF) and aqueous humor of the eye. In contrast, no PPT-A mRNA could be detected in samples from untreated animals. In addition, several neuropeptides (substance P, neuropeptide Y, cholecystokinin, calcitonin gene-related peptide and pituitary adenylate cyclase activating peptide) were released into the CSF (and aqueous humor) following ECT. The results suggest that PPT-A mRNA was released together with neuropeptides into the CSF and aqueous humor in response to ECT. Indeed, previous studies have suggested that neurons can release neuropeptide mRNAs and that neurons are capable of taking up and expressing foreign mRNA. If neuropeptide mRNA can be taken up and utilized by another neuronal population, it might explain instances when neurons display 'phenotypic switch', i.e. the transient expression of novel neuropeptides.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Aqueous Humor/metabolism, Brain/metabolism, Electroconvulsive Therapy, Neuropeptides/cerebrospinal fluid, Optic Nerve/metabolism, Protein Precursors/genetics, RNA, Messenger/cerebrospinal fluid, Rabbits, Tachykinins/genetics
in
Neuroscience Letters
volume
226
issue
3
pages
151 - 154
publisher
Elsevier
external identifiers
  • pmid:9175589
  • scopus:0030984925
ISSN
0304-3940
DOI
10.1016/s0304-3940(97)00272-3
language
English
LU publication?
yes
id
508cade8-5114-44e4-b8ec-c27cd32f7e60
date added to LUP
2019-09-03 13:56:41
date last changed
2024-01-01 18:33:59
@article{508cade8-5114-44e4-b8ec-c27cd32f7e60,
  abstract     = {{<p>Following electroconvulsive treatment (ECT) of rabbits, preprotachykinin-A (PPT-A) mRNA was detected by Southern blot analysis of polymerase chain reaction (PCR)-amplified products in the cerebrospinal fluid (CSF) and aqueous humor of the eye. In contrast, no PPT-A mRNA could be detected in samples from untreated animals. In addition, several neuropeptides (substance P, neuropeptide Y, cholecystokinin, calcitonin gene-related peptide and pituitary adenylate cyclase activating peptide) were released into the CSF (and aqueous humor) following ECT. The results suggest that PPT-A mRNA was released together with neuropeptides into the CSF and aqueous humor in response to ECT. Indeed, previous studies have suggested that neurons can release neuropeptide mRNAs and that neurons are capable of taking up and expressing foreign mRNA. If neuropeptide mRNA can be taken up and utilized by another neuronal population, it might explain instances when neurons display 'phenotypic switch', i.e. the transient expression of novel neuropeptides.</p>}},
  author       = {{Wang, Z Y and Monstein, H J and Waldeck, K and Grundemar, L and Håkanson, R}},
  issn         = {{0304-3940}},
  keywords     = {{Animals; Aqueous Humor/metabolism; Brain/metabolism; Electroconvulsive Therapy; Neuropeptides/cerebrospinal fluid; Optic Nerve/metabolism; Protein Precursors/genetics; RNA, Messenger/cerebrospinal fluid; Rabbits; Tachykinins/genetics}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{3}},
  pages        = {{151--154}},
  publisher    = {{Elsevier}},
  series       = {{Neuroscience Letters}},
  title        = {{Electroconvulsive treatment evokes release of preprotachykinin-A mRNA into the cerebrospinal fluid and ocular aqueous humor of rabbits}},
  url          = {{http://dx.doi.org/10.1016/s0304-3940(97)00272-3}},
  doi          = {{10.1016/s0304-3940(97)00272-3}},
  volume       = {{226}},
  year         = {{1997}},
}