Bioelectrochemical probing of intracellular redox processes in living yeast cells-application of redox polymer wiring in a microfluidic environment
(2013) In Analytical and Bioanalytical Chemistry 405(11). p.3847-3858- Abstract
- Conventionally, microbial bioelectrochemical assays have been conducted using immobilized cells on an electrode that is placed in an electrochemical batch cell. In this paper, we describe a developed microfluidic platform with integrated microelectrode arrays for automated bioelectrochemical assays utilizing a new double mediator system to map redox metabolism and screen for genetic modifications in Saccharomyces cerevisiae cells. The function of this new double mediator system based on menadione and osmium redox polymer (PVI-Os) is demonstrated. "Wiring" of S. cerevisiae cells using PVI-Os shows a significant improvement of bioelectrochemical monitoring in a microfluidic environment and functions as an effective immobilization matrix for... (More)
- Conventionally, microbial bioelectrochemical assays have been conducted using immobilized cells on an electrode that is placed in an electrochemical batch cell. In this paper, we describe a developed microfluidic platform with integrated microelectrode arrays for automated bioelectrochemical assays utilizing a new double mediator system to map redox metabolism and screen for genetic modifications in Saccharomyces cerevisiae cells. The function of this new double mediator system based on menadione and osmium redox polymer (PVI-Os) is demonstrated. "Wiring" of S. cerevisiae cells using PVI-Os shows a significant improvement of bioelectrochemical monitoring in a microfluidic environment and functions as an effective immobilization matrix for cells that are not strongly adherent. The function of the developed microfluidic platform is demonstrated using two strains of S. cerevisiae, ENY. WA and its deletion mutant EBY44, which lacks the enzyme phosphoglucose isomerase. The cellular responses to introduced glucose and fructose were recorded for the two S. cerevisiae strains, and the obtained results are compared with previously published work when using an electrochemical batch cell, indicating that microfluidic bioelectrochemical assays employing the menadione-PVI-Os double mediator system provides an effective means to conduct automated microbial assays. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3854779
- author
- Heiskanen, Arto ; Coman, Vasile ; Kostesha, Natalie ; Sabourin, David ; Haslett, Nick ; Baronian, Keith ; Gorton, Lo LU ; Dufva, Martin and Emneus, Jenny
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cellular redox activity, Microbial bioelectrochemistry, Osmium redox, polymer, Double mediator system, Saccharomyces cerevisiae, Microfluidic, system
- in
- Analytical and Bioanalytical Chemistry
- volume
- 405
- issue
- 11
- pages
- 3847 - 3858
- publisher
- Springer
- external identifiers
-
- wos:000317643100037
- scopus:84877135369
- pmid:23371527
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-013-6709-4
- language
- English
- LU publication?
- yes
- id
- 50aa93f4-47b1-490b-a632-5c8d675ebbe0 (old id 3854779)
- date added to LUP
- 2016-04-01 10:28:59
- date last changed
- 2023-08-31 04:13:43
@article{50aa93f4-47b1-490b-a632-5c8d675ebbe0,
abstract = {{Conventionally, microbial bioelectrochemical assays have been conducted using immobilized cells on an electrode that is placed in an electrochemical batch cell. In this paper, we describe a developed microfluidic platform with integrated microelectrode arrays for automated bioelectrochemical assays utilizing a new double mediator system to map redox metabolism and screen for genetic modifications in Saccharomyces cerevisiae cells. The function of this new double mediator system based on menadione and osmium redox polymer (PVI-Os) is demonstrated. "Wiring" of S. cerevisiae cells using PVI-Os shows a significant improvement of bioelectrochemical monitoring in a microfluidic environment and functions as an effective immobilization matrix for cells that are not strongly adherent. The function of the developed microfluidic platform is demonstrated using two strains of S. cerevisiae, ENY. WA and its deletion mutant EBY44, which lacks the enzyme phosphoglucose isomerase. The cellular responses to introduced glucose and fructose were recorded for the two S. cerevisiae strains, and the obtained results are compared with previously published work when using an electrochemical batch cell, indicating that microfluidic bioelectrochemical assays employing the menadione-PVI-Os double mediator system provides an effective means to conduct automated microbial assays.}},
author = {{Heiskanen, Arto and Coman, Vasile and Kostesha, Natalie and Sabourin, David and Haslett, Nick and Baronian, Keith and Gorton, Lo and Dufva, Martin and Emneus, Jenny}},
issn = {{1618-2642}},
keywords = {{Cellular redox activity; Microbial bioelectrochemistry; Osmium redox; polymer; Double mediator system; Saccharomyces cerevisiae; Microfluidic; system}},
language = {{eng}},
number = {{11}},
pages = {{3847--3858}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Bioelectrochemical probing of intracellular redox processes in living yeast cells-application of redox polymer wiring in a microfluidic environment}},
url = {{http://dx.doi.org/10.1007/s00216-013-6709-4}},
doi = {{10.1007/s00216-013-6709-4}},
volume = {{405}},
year = {{2013}},
}