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Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

Uhlin, Elias ; Rönnholm, Harriet ; Day, Kelly ; Kele, Malin ; Tammimies, Kristiina ; Bölte, Sven and Falk, Anna LU (2017) In Stem Cell Research 18. p.22-25
Abstract

Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Line, Cellular Reprogramming, Culture Media/chemistry, Fibroblasts/cytology, Genetic Vectors/genetics, Humans, Induced Pluripotent Stem Cells/cytology, Karyotype, Male, RNA, Messenger/metabolism, Sendai virus/genetics, Transcription Factors/genetics, Twins, Monozygotic
in
Stem Cell Research
volume
18
pages
4 pages
publisher
Elsevier
external identifiers
  • pmid:28395796
  • scopus:85024398865
ISSN
1876-7753
DOI
10.1016/j.scr.2016.12.006
language
English
LU publication?
no
id
50c03091-47d2-4ab2-bdaf-8bb777ae4f7e
date added to LUP
2021-08-10 12:45:23
date last changed
2024-05-18 12:52:14
@article{50c03091-47d2-4ab2-bdaf-8bb777ae4f7e,
  abstract     = {{<p>Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.</p>}},
  author       = {{Uhlin, Elias and Rönnholm, Harriet and Day, Kelly and Kele, Malin and Tammimies, Kristiina and Bölte, Sven and Falk, Anna}},
  issn         = {{1876-7753}},
  keywords     = {{Cell Line; Cellular Reprogramming; Culture Media/chemistry; Fibroblasts/cytology; Genetic Vectors/genetics; Humans; Induced Pluripotent Stem Cells/cytology; Karyotype; Male; RNA, Messenger/metabolism; Sendai virus/genetics; Transcription Factors/genetics; Twins, Monozygotic}},
  language     = {{eng}},
  pages        = {{22--25}},
  publisher    = {{Elsevier}},
  series       = {{Stem Cell Research}},
  title        = {{Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions}},
  url          = {{https://lup.lub.lu.se/search/files/101075172/Derivation_of_human_iPS_cell.pdf}},
  doi          = {{10.1016/j.scr.2016.12.006}},
  volume       = {{18}},
  year         = {{2017}},
}