Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions
(2017) In Stem Cell Research 18. p.22-25- Abstract
Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/50c03091-47d2-4ab2-bdaf-8bb777ae4f7e
- author
- Uhlin, Elias ; Rönnholm, Harriet ; Day, Kelly ; Kele, Malin ; Tammimies, Kristiina ; Bölte, Sven and Falk, Anna LU
- publishing date
- 2017-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell Line, Cellular Reprogramming, Culture Media/chemistry, Fibroblasts/cytology, Genetic Vectors/genetics, Humans, Induced Pluripotent Stem Cells/cytology, Karyotype, Male, RNA, Messenger/metabolism, Sendai virus/genetics, Transcription Factors/genetics, Twins, Monozygotic
- in
- Stem Cell Research
- volume
- 18
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:28395796
- scopus:85024398865
- ISSN
- 1876-7753
- DOI
- 10.1016/j.scr.2016.12.006
- language
- English
- LU publication?
- no
- id
- 50c03091-47d2-4ab2-bdaf-8bb777ae4f7e
- date added to LUP
- 2021-08-10 12:45:23
- date last changed
- 2024-09-22 22:25:59
@article{50c03091-47d2-4ab2-bdaf-8bb777ae4f7e, abstract = {{<p>Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.</p>}}, author = {{Uhlin, Elias and Rönnholm, Harriet and Day, Kelly and Kele, Malin and Tammimies, Kristiina and Bölte, Sven and Falk, Anna}}, issn = {{1876-7753}}, keywords = {{Cell Line; Cellular Reprogramming; Culture Media/chemistry; Fibroblasts/cytology; Genetic Vectors/genetics; Humans; Induced Pluripotent Stem Cells/cytology; Karyotype; Male; RNA, Messenger/metabolism; Sendai virus/genetics; Transcription Factors/genetics; Twins, Monozygotic}}, language = {{eng}}, pages = {{22--25}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions}}, url = {{https://lup.lub.lu.se/search/files/101075172/Derivation_of_human_iPS_cell.pdf}}, doi = {{10.1016/j.scr.2016.12.006}}, volume = {{18}}, year = {{2017}}, }