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A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography

Lovric, Goran; Mokso, Rajmund LU ; Schlepütz, Christian M. and Stampanoni, Marco (2016) In Physica Medica 32(12). p.1771-1778
Abstract

Time-resolved imaging of dynamic processes, ranging from biological in vivo studies to materials under in situ and in operando conditions, requires a flexible endstation capable of controlling complex components that interact in different configurations and at high speeds. At the X02DA TOMCAT beamline we have recently achieved in situ tomographic measurements at a rate of 20 Hz. Independently, we have shown the feasibility of in vivo lung imaging down to the micrometer scale. In the present paper, we discuss the latest developments in view of instrumentation and the accompanying components for achieving these two types of measurements. As the prime example, we focus on the technical requirements for in vivo tomographic microscopy of the... (More)

Time-resolved imaging of dynamic processes, ranging from biological in vivo studies to materials under in situ and in operando conditions, requires a flexible endstation capable of controlling complex components that interact in different configurations and at high speeds. At the X02DA TOMCAT beamline we have recently achieved in situ tomographic measurements at a rate of 20 Hz. Independently, we have shown the feasibility of in vivo lung imaging down to the micrometer scale. In the present paper, we discuss the latest developments in view of instrumentation and the accompanying components for achieving these two types of measurements. As the prime example, we focus on the technical requirements for in vivo tomographic microscopy of the lung at the micrometer scale in terms of acquisition schemes, triggering and radiation dose. We identify ultra-short single-projection exposures combined with accurate triggering capabilities as the main prerequisites to obtain high-quality reconstructions while limiting the X-ray dose imparted on the living sample. The presented endstation offers generic high-speed imaging capabilities, as it is compatible with a variety of experimental setups and suitable for a wide range of time-resolved studies.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
In vivo, Time-resolved tomography 2010, X-ray imaging
in
Physica Medica
volume
32
issue
12
pages
8 pages
publisher
ISTITUTI EDITORIALI E POLGRAFICI INTERNAZIONALI
external identifiers
  • scopus:85005978362
  • wos:000390597500040
ISSN
1120-1797
DOI
10.1016/j.ejmp.2016.08.012
language
English
LU publication?
yes
id
5106e773-0740-4606-bbf5-64c6b2be7bf1
date added to LUP
2017-01-25 11:17:17
date last changed
2017-11-12 04:28:23
@article{5106e773-0740-4606-bbf5-64c6b2be7bf1,
  abstract     = {<p>Time-resolved imaging of dynamic processes, ranging from biological in vivo studies to materials under in situ and in operando conditions, requires a flexible endstation capable of controlling complex components that interact in different configurations and at high speeds. At the X02DA TOMCAT beamline we have recently achieved in situ tomographic measurements at a rate of 20 Hz. Independently, we have shown the feasibility of in vivo lung imaging down to the micrometer scale. In the present paper, we discuss the latest developments in view of instrumentation and the accompanying components for achieving these two types of measurements. As the prime example, we focus on the technical requirements for in vivo tomographic microscopy of the lung at the micrometer scale in terms of acquisition schemes, triggering and radiation dose. We identify ultra-short single-projection exposures combined with accurate triggering capabilities as the main prerequisites to obtain high-quality reconstructions while limiting the X-ray dose imparted on the living sample. The presented endstation offers generic high-speed imaging capabilities, as it is compatible with a variety of experimental setups and suitable for a wide range of time-resolved studies.</p>},
  author       = {Lovric, Goran and Mokso, Rajmund and Schlepütz, Christian M. and Stampanoni, Marco},
  issn         = {1120-1797},
  keyword      = {In vivo,Time-resolved tomography 2010,X-ray imaging},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {1771--1778},
  publisher    = {ISTITUTI EDITORIALI E POLGRAFICI INTERNAZIONALI},
  series       = {Physica Medica},
  title        = {A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography},
  url          = {http://dx.doi.org/10.1016/j.ejmp.2016.08.012},
  volume       = {32},
  year         = {2016},
}