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Retinoic Acid regulates hematopoietic development from human pluripotent stem cells.

Rönn, Roger LU ; Guibentif, Carolina LU ; Moraghebi, Roksana LU ; Chaves Guerrero, Patricia LU ; Saxena, Shobhit LU ; Garcia, Bradley and Woods, Niels-Bjarne LU (2015) In Stem Cell Reports 4(2). p.269-281
Abstract
The functions of retinoic acid (RA), a potent morphogen with crucial roles in embryogenesis including developmental hematopoiesis, have not been thoroughly investigated in the human setting. Using an in vitro model of human hematopoietic development, we evaluated the effects of RA signaling on the development of blood and on generated hematopoietic progenitors. Decreased RA signaling increases the generation of cells with a hematopoietic stem cell (HSC)-like phenotype, capable of differentiation into myeloid and lymphoid lineages, through two separate mechanisms: by increasing the commitment of pluripotent stem cells toward the hematopoietic lineage during the developmental process and by decreasing the differentiation of generated blood... (More)
The functions of retinoic acid (RA), a potent morphogen with crucial roles in embryogenesis including developmental hematopoiesis, have not been thoroughly investigated in the human setting. Using an in vitro model of human hematopoietic development, we evaluated the effects of RA signaling on the development of blood and on generated hematopoietic progenitors. Decreased RA signaling increases the generation of cells with a hematopoietic stem cell (HSC)-like phenotype, capable of differentiation into myeloid and lymphoid lineages, through two separate mechanisms: by increasing the commitment of pluripotent stem cells toward the hematopoietic lineage during the developmental process and by decreasing the differentiation of generated blood progenitors. Our results demonstrate that controlled low-level RA signaling is a requirement in human blood development, and we propose a new interpretation of RA as a regulatory factor, where appropriate control of RA signaling enables increased generation of hematopoietic progenitor cells from pluripotent stem cells in vitro. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Reports
volume
4
issue
2
pages
269 - 281
publisher
Cell Press
external identifiers
  • pmid:25680478
  • wos:000349629000009
  • scopus:84922467678
ISSN
2213-6711
DOI
10.1016/j.stemcr.2015.01.009
language
English
LU publication?
yes
id
366dc888-b78f-4b0a-896e-5a30e53ee4c8 (old id 5143757)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25680478?dopt=Abstract
date added to LUP
2015-03-07 14:32:28
date last changed
2017-07-02 04:07:51
@article{366dc888-b78f-4b0a-896e-5a30e53ee4c8,
  abstract     = {The functions of retinoic acid (RA), a potent morphogen with crucial roles in embryogenesis including developmental hematopoiesis, have not been thoroughly investigated in the human setting. Using an in vitro model of human hematopoietic development, we evaluated the effects of RA signaling on the development of blood and on generated hematopoietic progenitors. Decreased RA signaling increases the generation of cells with a hematopoietic stem cell (HSC)-like phenotype, capable of differentiation into myeloid and lymphoid lineages, through two separate mechanisms: by increasing the commitment of pluripotent stem cells toward the hematopoietic lineage during the developmental process and by decreasing the differentiation of generated blood progenitors. Our results demonstrate that controlled low-level RA signaling is a requirement in human blood development, and we propose a new interpretation of RA as a regulatory factor, where appropriate control of RA signaling enables increased generation of hematopoietic progenitor cells from pluripotent stem cells in vitro.},
  author       = {Rönn, Roger and Guibentif, Carolina and Moraghebi, Roksana and Chaves Guerrero, Patricia and Saxena, Shobhit and Garcia, Bradley and Woods, Niels-Bjarne},
  issn         = {2213-6711},
  language     = {eng},
  number       = {2},
  pages        = {269--281},
  publisher    = {Cell Press},
  series       = {Stem Cell Reports},
  title        = {Retinoic Acid regulates hematopoietic development from human pluripotent stem cells.},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2015.01.009},
  volume       = {4},
  year         = {2015},
}