The Masquelet Induced Membrane Technique with BMP and a Synthetic Scaffold Can Heal a Rat Femoral Critical Size Defect.
(2015) In Journal of Orthopaedic Research 33(4). p.488-495- Abstract
- Long bone defects can be managed by the induced membrane technique together with autologous bone graft. However, graft harvest is associated with donor site morbidity. This study investigates if a tricalcium phosphate hydroxyapatite scaffold can be used alone or in combination with bone active drugs to improve healing. Sprague Dawley rats (n = 40) were randomized into four groups. (A) scaffold, (B) BMP-7, (C) BMP-7 + scaffold, and (D) BMP-7 + scaffold + systemic bisphosphonate at 2 weeks. Locked femoral nailing was followed by 6 mm segment removal and implantation of an epoxy spacer. At 4 weeks, the spacers were removed and the defects grafted. Eleven weeks later, the bones were explanted for evaluation with radiography, manual assessment,... (More)
- Long bone defects can be managed by the induced membrane technique together with autologous bone graft. However, graft harvest is associated with donor site morbidity. This study investigates if a tricalcium phosphate hydroxyapatite scaffold can be used alone or in combination with bone active drugs to improve healing. Sprague Dawley rats (n = 40) were randomized into four groups. (A) scaffold, (B) BMP-7, (C) BMP-7 + scaffold, and (D) BMP-7 + scaffold + systemic bisphosphonate at 2 weeks. Locked femoral nailing was followed by 6 mm segment removal and implantation of an epoxy spacer. At 4 weeks, the spacers were removed and the defects grafted. Eleven weeks later, the bones were explanted for evaluation with radiography, manual assessment, micro-CT, histology, and Fourier Transform Infrared spectroscopy (FTIR). Isolated scaffolds (A) did not heal any defects, whereas the other treatments led to healing in 7/10 (B), 10/10 (C), and 9/10 (D) rats. Group D had greater volume of highly mineralized bone (p < 0.01) and higher bone volume fraction (p < 0.01) compared to all other groups. A synthetic scaffold + BMP-7 combined with a bisphosphonate improved the callus properties in a rat femoral critical size defect, compared to both BMP-7 and scaffold alone or the two combined. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5145732
- author
- Bosemark, Per LU ; Perdikouri, Christina LU ; Pelkonen, Mea LU ; Isaksson, Hanna and Tägil, Magnus LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Orthopaedic Research
- volume
- 33
- issue
- 4
- pages
- 488 - 495
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:25639666
- wos:000351687400007
- scopus:84925649221
- pmid:25639666
- ISSN
- 1554-527X
- DOI
- 10.1002/jor.22815
- language
- English
- LU publication?
- yes
- id
- cfe4e23c-7ed9-41c6-b178-8644816c11e0 (old id 5145732)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25639666?dopt=Abstract
- date added to LUP
- 2016-04-01 10:09:48
- date last changed
- 2022-04-27 19:04:37
@article{cfe4e23c-7ed9-41c6-b178-8644816c11e0, abstract = {{Long bone defects can be managed by the induced membrane technique together with autologous bone graft. However, graft harvest is associated with donor site morbidity. This study investigates if a tricalcium phosphate hydroxyapatite scaffold can be used alone or in combination with bone active drugs to improve healing. Sprague Dawley rats (n = 40) were randomized into four groups. (A) scaffold, (B) BMP-7, (C) BMP-7 + scaffold, and (D) BMP-7 + scaffold + systemic bisphosphonate at 2 weeks. Locked femoral nailing was followed by 6 mm segment removal and implantation of an epoxy spacer. At 4 weeks, the spacers were removed and the defects grafted. Eleven weeks later, the bones were explanted for evaluation with radiography, manual assessment, micro-CT, histology, and Fourier Transform Infrared spectroscopy (FTIR). Isolated scaffolds (A) did not heal any defects, whereas the other treatments led to healing in 7/10 (B), 10/10 (C), and 9/10 (D) rats. Group D had greater volume of highly mineralized bone (p < 0.01) and higher bone volume fraction (p < 0.01) compared to all other groups. A synthetic scaffold + BMP-7 combined with a bisphosphonate improved the callus properties in a rat femoral critical size defect, compared to both BMP-7 and scaffold alone or the two combined. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.}}, author = {{Bosemark, Per and Perdikouri, Christina and Pelkonen, Mea and Isaksson, Hanna and Tägil, Magnus}}, issn = {{1554-527X}}, language = {{eng}}, number = {{4}}, pages = {{488--495}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Orthopaedic Research}}, title = {{The Masquelet Induced Membrane Technique with BMP and a Synthetic Scaffold Can Heal a Rat Femoral Critical Size Defect.}}, url = {{http://dx.doi.org/10.1002/jor.22815}}, doi = {{10.1002/jor.22815}}, volume = {{33}}, year = {{2015}}, }